DIG-HIGH PRIME 化学特性,用途語,生産方法
使用
DIG-High Prime-labeled DNA probes has been used in a variety of hybridization techniques :
- in Southern blots
- in northern blots
- in dot/slot blots
- for screening of gene libraries
- in In situ hybridizations
Due to highly specific and sensitive detection systems, DIG-labeled DNA probes can be used for single-copy gene detection in 1μg total human DNA. The use of the alkali-labile form of DIG-dUTP, in which the digoxigenin moiety is connected to the spacer arm via an alkali-labile ester bond, enables easier and more efficient stripping and reprobing of blots.
一般的な説明
DIG-High Prime has enzyme and nucleotide mixture for rapid random-primed labeling of DNA with Digoxigenin-11-dUTP. In this method, the complementary DNA strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. DIG-labeled probes are generated at high yield within one hour or after overnight incubation.
DIG-HIGH PRIME 上流と下流の製品情報
原材料
準備製品