EC 2.7.1.40
- CAS No.
- 9001-59-6
- Chemical Name:
- EC 2.7.1.40
- Synonyms
- PK;EC 2.7.1.40;pyruvic kinase;PYRUVATE KINASE;PK (rabbit muscle);Pyruvate Kinase M2;PK,pyruvate kinase;EC 2.7.1.40 USP/EP/BP;pyruvate phosphoferase;PYRUVATE KINASE TYPE I
- CBNumber:
- CB9396847
- Molecular Formula:
- NULL
- Molecular Weight:
- 0
- MDL Number:
- MFCD00081946
- MOL File:
- Mol file
- MSDS File:
- SDS
storage temp. | -20°C |
---|---|
form | powder |
color | white |
Water Solubility | Soluble in water ( 1mg/ml). |
EPA Substance Registry System | Kinase (phosphorylating), pyruvate (9001-59-6) |
SAFETY
Risk and Safety Statements
WGK Germany | 3 |
---|---|
F | 10-21 |
TSCA | Yes |
HS Code | 35079090 |
EC 2.7.1.40 price More Price(18)
Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
---|---|---|---|---|---|---|---|
Sigma-Aldrich | P9136 | Pyruvate Kinase from rabbit muscle Type III, lyophilized powder, 350-600?units/mg protein | 9001-59-6 | 1000units | $79.4 | 2024-03-01 | Buy |
Sigma-Aldrich | P9136 | Pyruvate Kinase from rabbit muscle Type III, lyophilized powder, 350-600?units/mg protein | 9001-59-6 | 5000units | $199 | 2024-03-01 | Buy |
Sigma-Aldrich | SRP0414 | PKLR Var2 active human recombinant,expressedinE.coli,≥90%(SDS-PAGE) | 9001-59-6 | 20μg | $653 | 2024-03-01 | Buy |
Sigma-Aldrich | SRP0413 | PKLR Var1 active human recombinant,expressedinE.coli,≥43%(SDS-PAGE) | 9001-59-6 | 20μg | $653 | 2024-03-01 | Buy |
Sigma-Aldrich | P9136 | Pyruvate Kinase from rabbit muscle Type III, lyophilized powder, 350-600?units/mg protein | 9001-59-6 | 25000units | $602 | 2024-03-01 | Buy |
EC 2.7.1.40 Chemical Properties,Uses,Production
Chemical Properties
White powder
Uses
Pyruvate kinase has been used in the preparation of cell free extract from BL21 CP strain of E. coli culture for highly productive cell-free protein expression. The enzyme has been used to assay the pyruvate kinase activity by incubating with eluted DAPk (Death-associated protein kinase), a serine/threonine kinase that binds and activates pyruvate kinase. It is involved in glycolysis and in gluconeogenesis. It has been used in PGM enzyme assays to determine phosphoglycerate mutase activity. It is also used to study pyruvate kinase (PK) deficiency.
Uses
Pyruvate kinase from Bacillus stearothermophilus has been used in a study to assess evidence that the genes for phosphofructokinase and pyruvate kinase constitute an operon. It has also been used in a study to investigate the importance of the Lys221 active site for pyruvate kinase activity.
Uses
Pyruvate kinase from rabbit muscle has been used in a structural study to understand the reaction mechanism of the final step in glycolysis. It has also been used in a study to investigate ATP-dependent phosphorylation of α-substituted carboxylic acids.
General Description
Pyruvate Kinase (PK) is a glycolysis enzyme and has four isozymes L, R, M1, and M2. The L isozyme is localized in gluconeogenic tissues, particularly in the liver. Whereas the R and M1 are localized in the adult skeletal muscles, heart, brain and erythrocytes, respectively. M2 is localized in the nucleus of the cells.
Biochem/physiol Actions
Molecular Weight: 237 kDa?and exists as a tetramer of four equal subunits of molecular weight 57 kDa.Isoelectric Point: 7.6Optimal pH: ~7.5 Optimal Temperature: 25°C ΕA280 = 0.54 for 1 mg(p)/ml, 1 cm path Reported KM values are ATP (0.86 mM), pyruvate (10 mM), ADP (0.3 mM), and PEP (0.07 mM) in Tris buffer at pH 7.4 and 30 °C. Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.
Purification Methods
These are purified by (NH4)2SO4 fractionation and gel filtration, ion-exchange and affinity chromatography. [Garcia-Olalla & Garrido-Pertierra Biochem J 241 573 1987.]
EC 2.7.1.40 Preparation Products And Raw materials
Supplier | Tel | Country | ProdList | Advantage | |
---|---|---|---|---|---|
Shanghai Zheyan Biotech Co., Ltd. | 18017610038 | zheyansh@163.com | CHINA | 3620 | 58 |
career henan chemical co | +86-0371-86658258 +8613203830695 | sales@coreychem.com | China | 29894 | 58 |
Chongqing Chemdad Co., Ltd | +86-023-6139-8061 +86-86-13650506873 | sales@chemdad.com | China | 39916 | 58 |
CONIER CHEM AND PHARMA LIMITED | +8618523575427 | sales@conier.com | China | 49391 | 58 |
Creative Enzymes | 1-516-855-7709 | info@creative-enzymes.com | United States | 8748 | 58 |
Hubei Xindesheng Material Technology Co., Ltd. | +8618971041571 | vickyzhao@whdschem.com | China | 174 | 58 |
Hefei TNJ Chemical Industry Co.,Ltd. | 0551-65418671 | sales@tnjchem.com | China | 34571 | 58 |
HANGZHOU CLAP TECHNOLOGY CO.,LTD | 86-571-88216897,88216896 13588875226 | sales@hzclap.com | CHINA | 6313 | 58 |
ANHUI WITOP BIOTECH CO., LTD | +8615255079626 | eric@witopchemical.com | China | 23556 | 58 |
Dideu Industries Group Limited | +86-29-89586680 +86-15129568250 | 1026@dideu.com | China | 24330 | 58 |
View Lastest Price from EC 2.7.1.40 manufacturers
Image | Update time | Product | Price | Min. Order | Purity | Supply Ability | Manufacturer | |
---|---|---|---|---|---|---|---|---|
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2024-08-22 | Pyruvate Kinase
9001-59-6
|
US $0.00-0.00 / g | 1g | 98.0% | 1kg/month | WUHAN FORTUNA CHEMICAL CO., LTD | |
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2023-08-03 | Pyruvate Kinase
9001-59-6
|
US $12.00-9.00 / KU | 10KU | ≥ 25.0U/mg | 10000ku | Hubei Xindesheng Material Technology Co., Ltd. | |
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2019-07-06 | carnosine
9001-59-6
|
US $1.00 / kg | 1kg | 95%-99% | 1000kg | Career Henan Chemical Co |
-
- Pyruvate Kinase
9001-59-6
- US $0.00-0.00 / g
- 98.0%
- WUHAN FORTUNA CHEMICAL CO., LTD
-
- Pyruvate Kinase
9001-59-6
- US $12.00-9.00 / KU
- ≥ 25.0U/mg
- Hubei Xindesheng Material Technology Co., Ltd.
-
- carnosine
9001-59-6
- US $1.00 / kg
- 95%-99%
- Career Henan Chemical Co
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