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DPN I

DPN I Structure
CAS No.
81295-14-9
Chemical Name:
DPN I
Synonyms
DPN I;DPN I RESTRICTION ENZYME;restriction endonuclease Dpn I;RESTRICTION ENDONUCLEASES DPN I;dpn i from diplococcus pneumoniae;restriction endonuclease dpn I from*diplococcus P;RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE
CBNumber:
CB0256555
Molecular Formula:
Molecular Weight:
0
MOL File:
Mol file
Modify Date:
2023/4/23 13:52:06
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DPN I Properties

storage temp. -20°C
form buffered aqueous glycerol solution

SAFETY

Risk and Safety Statements

RIDADR  UN3373 - class 6.2 Biological substance, Category B
WGK Germany  3

DPN I Chemical Properties,Uses,Production

Uses

In PCR-mediated mutagenesis, DpnI nuclease has been used for the digestion of wild type DNA after PCR.

General Description

Isoschizomers
The enzyme is an isoschizomer to Bsp143 I, Dpn II, Mbo I, Nde II and Sau3A I.
Methylation sensitivity
The enzyme is only inhibited by the occurrence of 5-methylcytosine at the indicated site (*) if no 6-methyladenine is present.
Typical ligation and recutting assay
Dpn I fragments obtained by complete digestion of 1μg pBR322 DNA are ligated for 16 hours at +4°C with 1U T4 DNA Ligase in 10μl buffer that contains 66mM Tris-HCl, 5mM MgCl2, 5mM Dithiothreitol, 1mM ATP, pH 7.5 (at +20°C). The percentages of product that can be ligated and subsequently recut with Dpn I (yielding the typical pattern of pBR322 × Dpn I fragments) are stated under "Lig" and "Rec" in the certificate of analysis.

DPN I Preparation Products And Raw materials

Raw materials

Preparation Products

DPN I Suppliers

Global( 60)Suppliers
Supplier Tel Country ProdList Advantage Inquiry
Career Henan Chemica Co +86-0371-86658258 +8613203830695 China 30253 58 Inquiry
Shanghai Mengze Biotechnology Co., Ltd. 13817203524 CHINA 6393 58 Inquiry
Sigma-Aldrich 021-61415566 800-8193336 China 51471 80 Inquiry
Shanghai Yuchun Biotechnology Co., Ltd 021-021-52273538 17321241593 China 5255 58 Inquiry
Wuhan Chemstan Biotechnology Co., Ltd. 027-65317797 15926423062 China 10036 58 Inquiry
Beyotime Biotechnology 021-67602129 CHINA 4658 58 Inquiry
MP Biomedicals, Inc. 949 833 2500 United States 6566 81 Inquiry
FERMENTAS Inc. 1 800 340 9026 United States 335 82 Inquiry
Roche Applied Science 800 428 5433 United States 696 85 Inquiry
Promega Corporation 1 800 356-9526 United States 612 82 Inquiry
Supplier Advantage
Career Henan Chemica Co 58
Shanghai Mengze Biotechnology Co., Ltd. 58
Sigma-Aldrich 80
Shanghai Yuchun Biotechnology Co., Ltd 58
Wuhan Chemstan Biotechnology Co., Ltd. 58
Beyotime Biotechnology 58
MP Biomedicals, Inc. 81
FERMENTAS Inc. 82
Roche Applied Science 85
Promega Corporation 82

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RESTRICTION ENDONUCLEASES DPN I DPN I DPN I RESTRICTION ENZYME restriction endonuclease dpn I from*diplococcus P RESTRICTION ENDONUCLEASE DPN I FROM*DIPL OCOCCUS PNE dpn i from diplococcus pneumoniae restriction endonuclease Dpn I 81295-14-9 Restriction Endonucleases Molecular Biology Enzymes Molecular Biology BioChemical Restriction EnzymesEnzyme Class Index 3.1.x.x Acting on esters 3.x.x.x Hydrolases Molecular Biology Molecular Biology Enzymes Molecular Biology Tested Restriction EndonucleasesApplication Index