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5(6)-Carboxyfluorescein

5(6)-Carboxyfluorescein Structure
CAS No.
72088-94-9
Chemical Name:
5(6)-Carboxyfluorescein
Synonyms
5(6)-FAM;DCFA;5(6)-Carboxyfluoresc;5(6)-CARBOXYLUORESCEIN;4(5)-CARBOXYFLUORESCEIN;5(6)-CARBOXYFLUORESCEIN;5(6)-Carboxyfluoresceine];4(5)-Carboxyfluoresceine];Fluoresceincarboxylic Acid;5(6)-CARBOXYFLUORESCEIN 99%
CBNumber:
CB5257173
Molecular Formula:
C42H24O14
Molecular Weight:
752.63
MOL File:
72088-94-9.mol
MSDS File:
SDS
Modify Date:
2024/6/24 22:20:23

5(6)-Carboxyfluorescein Properties

Melting point 275 °C
Boiling point 464.9°C (rough estimate)
Density 1.3418 (rough estimate)
refractive index 1.6380 (estimate)
storage temp. Keep in dark place,Inert atmosphere,Room temperature
solubility DMSO (Sparingly), Methanol (Slightly, Heated)
form Solid
color Light Yellow to Dark Brown
Water Solubility slightly soluble
InChIKey CPRVUBZYHZGASQ-UHFFFAOYSA-N
SMILES C12(OC(=O)C3C=CC(C(=O)O)=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1.C12(OC(=O)C3C=C(C(=O)O)C=CC1=3)C1=CC=C(O)C=C1OC1C=C(O)C=CC2=1
EPA Substance Registry System Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo- (72088-94-9)

SAFETY

Risk and Safety Statements

Symbol(GHS) 
GHS07
Signal word  Warning
Hazard statements  H319
Precautionary statements  P305+P351+P338
Hazard Codes  Xi,Xn
Risk Statements  36-36/37/38-20/21/22
Safety Statements  37/39-26-36-22
WGK Germany  3
TSCA  Yes
HS Code  29322090
NFPA 704
0
1 0

5(6)-Carboxyfluorescein price More Price(8)

Manufacturer Product number Product description CAS number Packaging Price Updated Buy
Sigma-Aldrich(India) 8.51082 5(6)-Carboxyfluorescein Novabiochem? 72088-94-9 1G ₹3080.01 2022-06-14 Buy
Sigma-Aldrich(India) 8.51082 5(6)-Carboxyfluorescein Novabiochem? 72088-94-9 5G ₹12540 2022-06-14 Buy
Sigma-Aldrich(India) 447293 5(6)-Carboxyfluorescein Dye content 90?% 72088-94-9 1G ₹7881 2022-06-14 Buy
Sigma-Aldrich(India) 447293 5(6)-Carboxyfluorescein Dye content 90?% 72088-94-9 5G ₹25929.6 2022-06-14 Buy
Sigma-Aldrich(India) 21877 5(6)-Carboxyfluorescein BioReagent, suitable for fluorescence, ≥95% (HPLC) 72088-94-9 1G ₹5283.6 2022-06-14 Buy
Product number Packaging Price Buy
8.51082 1G ₹3080.01 Buy
8.51082 5G ₹12540 Buy
447293 1G ₹7881 Buy
447293 5G ₹25929.6 Buy
21877 1G ₹5283.6 Buy

5(6)-Carboxyfluorescein Chemical Properties,Uses,Production

Description

5(6)-Carboxyfluorescein is a mixture of 5-carboxy and 6-carboxy derivatives of fluorescein. It is commonly used to label biomolecules through a reaction involving the carboxyl group. Unlike its succinimidyl ester derivative CFSE , 5(6)-carboxyfluorescein is membrane impermeant. As a result, it can be used in studies of membrane permeability. 5(6)-Carboxyfluorescein displays excellent fluorescence (excitation/emission at 492/514 nm, respectively), and its excitation maximum closely matches the 488 nm spectral line of argon-ion lasers.

Chemical Properties

Dark-Orange to Brown Solid

Uses

As a popular probe of determination of intracellular pH, 5(6)-Carboxyfluoresceincan be used as useful reagent for the preparation of hydrolytically stable fluorescent conjugates and a useful starting material for the synthesis of other fluorescein-derives reagent

Solubility in organics

5(6)-Carboxyfluorescein is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide. The solubility of 5(6)-carboxyfluorescein in these solvents is approximately 5, 0.5, and 1 mg/ml, respectively.

5(6)-Carboxyfluorescein Preparation Products And Raw materials

Raw materials

Preparation Products

Global( 241)Suppliers
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CLEARSYNTH LABS LTD. +91-22-45045900 Hyderabad, India 6351 58 Inquiry
A.J Chemicals 91-9810153283 New Delhi, India 6124 58 Inquiry
Alfa Aesar 1 800 209 7001 Maharashtra, India 6913 58 Inquiry
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Hongxiong +undefined18915499068 China 120 58 Inquiry
Hebei Mojin Biotechnology Co., Ltd +86 13288715578 +8613288715578 China 12447 58 Inquiry
Hebei Yanxi Chemical Co., Ltd. +86-17531190177; +8617531190177 China 5873 58 Inquiry
Hebei Kingfiner Technology Development Co.Ltd +86-15532196582 +86-15373005021 China 2989 58 Inquiry
Henan Fengda Chemical Co., Ltd +86-371-86557731 +86-13613820652 China 20294 58 Inquiry
hebei hongtan Biotechnology Co., Ltd +86-86-1913198-3935 +8617331935328 China 973 58 Inquiry
9’-[9h]xanthene]-ar-carboxylicacid,3’,6’-dihydroxy-spiro[isobenzofuran-1(3h 5-(AND-6)-CARBOXYFLUORESCEIN 5(6)-CARBOXYFLUORESCEIN 4(5)-CARBOXYFLUORESCEIN 3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid 5(6)-CARBOXYFLUORESCEIN, FOR FLUORESCENC E 5(6)-CARBOXYFLUORESCEIN 99% 5(6)-CARBOXYLUORESCEIN 4(5)-Carboxyfluoresceine] 5(6)-Carboxyfluoresceine] 5(6)-CARBOXYFLUORESCEIN 95+% 3',6'-Dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5(6)-carboxylic acid 5(6)-Carboxyfluorescein, mixture of isomers, 97% Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo- 5(6)-Carboxy-fluorescein, isomer mixture 5(6)-Carboxyfluorescein 5GR Fluoresceincarboxylic Acid 5(6)-Carboxyfluoresc 5(6)-Carboxyfluorescein - mixture 5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9* 5(6)-FAM [5-(and-6)-Carboxyfluorescein 5(6)-Carboxyfluorescein USP/EP/BP 3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthe... 5(6)-FAM DCFA 5(6)-FAM(5(6)-carboxyfluorescein) 72088-94-9 C22H16O7 C21H12O7 C42H24O14 Biochemicals and Reagents BioChemical Fluorescent Probes, Labels, Particles and Stains Detection marker Fluorescent Labels & Indicators Electronic Chemicals FLUOROPHORES