Fluorescein diacetate
- CAS No.
- 596-09-8
- Chemical Name:
- Fluorescein diacetate
- Synonyms
- FDA;Mfcd5062;Nsc 4726;Nsc 667259;Diacetylfluor;-Cellstain-Fda;fluoresceinacetate;DIACETYLFLUORESCEIN;3,6-DIACETOXYFLUORAN;Flomoxef Impurity 45
- CBNumber:
- CB5305799
- Molecular Formula:
- C24H16O7
- Molecular Weight:
- 416.38
- MOL File:
- 596-09-8.mol
- Modify Date:
- 2023/4/23 13:52:06
| Melting point | 200-203 °C(lit.) |
|---|---|
| Boiling point | 494.73°C (rough estimate) |
| Density | 1.2738 (rough estimate) |
| refractive index | 1.6810 (estimate) |
| storage temp. | −20°C |
| solubility | acetone: soluble25mg/mL |
| form | Crystalline Powder |
| pka | 6.4(at 25℃) |
| color | Light yellow |
| PH Range | Weak green uorescence (6.0) to strong green uorescence (7.2) |
| Water Solubility | Soluble in chloroform, DMSO, ethanol, and water. |
| λmax | 490nm |
| BRN | 62575 |
| Major Application | LED devices, petroleum markers, identifying cancerous cells, detecting bacteria, virus, mitochondrial function in neurons, target molecules, living cells, viable Giardia cysts in water samples, apoptosis, evaluating live/dead state of algae, measurement of totalmicrobial activity in soil, treating ischemia |
| Biological Applications | Assessing membrane integrity/permeability;43–50 detecting/counting microorganisms; measuring microbial activity; as a substrate for measuring acylases activity, amidases activity,aminopeptidases activity, arylsulfatases activity,chymotrypsin activity, esterases activity;galactosidases activity, glucosidases activity, lipases activity, phosphatases activity, proteases activity; cell viability assay; cytotoxicity assay; implantable medical devices; as temperature sensor; treating ischemic disease |
| CAS DataBase Reference | 596-09-8(CAS DataBase Reference) |
| EPA Substance Registry System | Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)- (596-09-8) |
SAFETY
Risk and Safety Statements
| Symbol(GHS) |  GHS07 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Signal word | Warning | |||||||||
| Hazard statements | H315-H319 | |||||||||
| Precautionary statements | P264-P280-P302+P352-P337+P313-P305+P351+P338-P362+P364-P332+P313 | |||||||||
| Hazard Codes | Xi | |||||||||
| Risk Statements | 36/37/38 | |||||||||
| Safety Statements | 24/25-36/37/39-27-26 | |||||||||
| WGK Germany | 3 | |||||||||
| TSCA | Yes | |||||||||
| HS Code | 32129000 | |||||||||
| NFPA 704 |
|
Fluorescein diacetate price More Price(6)
| Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
|---|---|---|---|---|---|---|---|
| Sigma-Aldrich(India) | F7378 | Fluorescein diacetate used as cell viability stain | 596-09-8 | 5G | ₹5760.9 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | F7378 | Fluorescein diacetate used as cell viability stain | 596-09-8 | 10G | ₹10656 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | F7378 | Fluorescein diacetate used as cell viability stain | 596-09-8 | 25G | ₹20124.3 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | F7378 | Fluorescein diacetate used as cell viability stain | 596-09-8 | 100G | ₹57453.6 | 2022-06-14 | Buy |
| Sigma-Aldrich(India) | 343209 | Fluorescein Diacetate - CAS 596-09-8 - Calbiochem Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases res | 596-09-8 | 5G | ₹11419.99 | 2022-06-14 | Buy |
Fluorescein diacetate Chemical Properties,Uses,Production
Description
Fluorescein diacetate (FDA) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, this AM ester yields fluorescein.
Chemical Properties
Light yellow powder
Uses
Fluorescein diacetate is a non-fluorescent molecule, which is hydrolysed to fluorescent fluorescein in live cells. Dead cells cannot accumulate or hydrolyse FDA. It has been used for the differentiation of live cells from dead cells. It can be also used as a fluorogenic substrate for hGSTP1-1.
Application
Diacetylfluorescein is used in fluorometric staining in the imaging of mammalian cells. Also used in the synthesis of nanocrystal biolabels with releasable fluorophores used in immunoassays. Dyes and metabolites.
Biotechnological Applications
Fluorescein Diacetate Stain is an esterase substrate that can permeate cell membranes. In viable cells with an active metabolism, intracellular esterases hydrolyze Fluorescein Diacetate Stain to produce the green fluorescent compound fluorescein. Fluorescein accumulates in viable cells, causing them to fluoresce green.
Fluorescein Diacetate Stain can be used with Propidium Iodide Stain for Yeast to assess cell viability with the automated fluorescence cell counters of the LUNA® family. Viable cells convert fluorescein diacetate into fluorescein and thus fluoresce green. Nonviable cells take up propidium iodide and fluoresce red.
Fluorescence Signal Enhancer 1 can be used with Fluorescein Diacetate Stain to inhibit ATP-dependent fluorescein efflux from metabolically active cells. This will result in lower background and increased signal intensity, thereby enhancing the fluorescein signal-to-noise ratio.
Fluorescein diacetate Preparation Products And Raw materials
Raw materials
Preparation Products
| Supplier | Tel | Country | ProdList | Advantage | Inquiry |
|---|---|---|---|---|---|
| Dhara Industries | +91-9322395199 +91-9322395199 | Mumbai, India | 190 | 58 | Inquiry |
| CLEARSYNTH LABS LTD. | +91-22-45045900 | Hyderabad, India | 6351 | 58 | Inquiry |
| A.J Chemicals | 91-9810153283 | New Delhi, India | 6124 | 58 | Inquiry |
| Alfa Aesar | 1 800 209 7001 | Maharashtra, India | 6913 | 58 | Inquiry |
| Central Drug House(P) Ltd. | 91-11-49404040 | New Delhi, India | 6160 | 58 | Inquiry |
| Pharmaffiliates Analytics and Synthetics P. Ltd | +91-172-5066494 | Haryana, India | 6773 | 58 | Inquiry |
| Indian Platinum Pvt.Ltd | 08048615427 | Mumbai, India | 113 | 58 | Inquiry |
| Unisource Chemicals Private Limited | 08048372772Ext 301 | Maharashtra, India | 290 | 58 | Inquiry |
| Molychem | 08048372666Ext 559 | Mumbai, India | 577 | 58 | Inquiry |
| Vyas Bio Life Sciences Pvt. Ltd | -65546373 | Hyderabad, India | 1213 | 58 | Inquiry |
| Supplier | Advantage |
|---|---|
| Dhara Industries | 58 |
| CLEARSYNTH LABS LTD. | 58 |
| A.J Chemicals | 58 |
| Alfa Aesar | 58 |
| Central Drug House(P) Ltd. | 58 |
| Pharmaffiliates Analytics and Synthetics P. Ltd | 58 |
| Indian Platinum Pvt.Ltd | 58 |
| Unisource Chemicals Private Limited | 58 |
| Molychem | 58 |
| Vyas Bio Life Sciences Pvt. Ltd | 58 |
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