플루오레센 다이아세테이트
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플루오레센 다이아세테이트 속성
- 녹는점
- 200-203 °C(lit.)
- 끓는 점
- 494.73°C (rough estimate)
- 밀도
- 1.2738 (rough estimate)
- 굴절률
- 1.6810 (estimate)
- 저장 조건
- −20°C
- 용해도
- 아세톤: 가용성25mg/mL
- 물리적 상태
- 결정성 분말
- 산도 계수 (pKa)
- 6.4(at 25℃)
- 색상
- 연노랑
- pH 범위
- Weak green uorescence (6.0) to strong green uorescence (7.2)
- 수용성
- 클로로포름, DMSO, 에탄올 및 물에 용해됩니다.
- 최대 파장(λmax)
- 490nm
- ε (흡광계수)
- ≥86000 at 488-494nm in 0.1 M NaOH
- BRN
- 62575
- 주요 응용
- LED devices, petroleum markers, identifying cancerous cells, detecting bacteria, virus, mitochondrial function in neurons, target molecules, living cells, viable Giardia cysts in water samples, apoptosis, evaluating live/dead state of algae, measurement of totalmicrobial activity in soil, treating ischemia
- 생물학 분야의 응용
- Assessing membrane integrity/permeability;43–50 detecting/counting microorganisms; measuring microbial activity; as a substrate for measuring acylases activity, amidases activity,aminopeptidases activity, arylsulfatases activity,chymotrypsin activity, esterases activity;galactosidases activity, glucosidases activity, lipases activity, phosphatases activity, proteases activity; cell viability assay; cytotoxicity assay; implantable medical devices; as temperature sensor; treating ischemic disease
- CAS 데이터베이스
- 596-09-8(CAS DataBase Reference)
안전
- 위험 및 안전 성명
- 위험 및 사전주의 사항 (GHS)
| 위험품 표기 | Xi | ||
|---|---|---|---|
| 위험 카페고리 넘버 | 36/37/38 | ||
| 안전지침서 | 24/25-36/37/39-27-26 | ||
| WGK 독일 | 3 | ||
| TSCA | Yes | ||
| HS 번호 | 32129000 |
플루오레센 다이아세테이트 C화학적 특성, 용도, 생산
개요
Fluorescein diacetate (FDA) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, this AM ester yields fluorescein.화학적 성질
Light yellow powder주요 응용
Diacetylfluorescein is used in fluorometric staining in the imaging of mammalian cells. Also used in the synthesis of nanocrystal biolabels with releasable fluorophores used in immunoassays. Dyes and metabolites.Biotechnological Applications
Fluorescein Diacetate Stain is an esterase substrate that can permeate cell membranes. In viable cells with an active metabolism, intracellular esterases hydrolyze Fluorescein Diacetate Stain to produce the green fluorescent compound fluorescein. Fluorescein accumulates in viable cells, causing them to fluoresce green.Fluorescein Diacetate Stain can be used with Propidium Iodide Stain for Yeast to assess cell viability with the automated fluorescence cell counters of the LUNA® family. Viable cells convert fluorescein diacetate into fluorescein and thus fluoresce green. Nonviable cells take up propidium iodide and fluoresce red.
Fluorescence Signal Enhancer 1 can be used with Fluorescein Diacetate Stain to inhibit ATP-dependent fluorescein efflux from metabolically active cells. This will result in lower background and increased signal intensity, thereby enhancing the fluorescein signal-to-noise ratio.
플루오레센 다이아세테이트 준비 용품 및 원자재
원자재
준비 용품
플루오레센 다이아세테이트 공급 업체
글로벌( 252)공급 업체
| 공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
|---|---|---|---|---|---|
| career henan chemical co | +86-0371-86658258 +8613203830695 |
sales@coreychem.com | China | 29879 | 58 |
| BOC Sciences | +1-631-485-4226 |
inquiry@bocsci.com | United States | 19553 | 58 |
| Chongqing Chemdad Co., Ltd | +86-023-6139-8061 +86-86-13650506873 |
sales@chemdad.com | China | 39894 | 58 |
| CONIER CHEM AND PHARMA LIMITED | +8618523575427 |
sales@conier.com | China | 49374 | 58 |
| Shaanxi Dideu Medichem Co. Ltd | +86-29-87569266 15319487004 |
1015@dideu.com | China | 3939 | 58 |
| TargetMol Chemicals Inc. | +1-781-999-5354 +1-00000000000 |
marketing@targetmol.com | United States | 32161 | 58 |
| Hefei TNJ Chemical Industry Co.,Ltd. | +86-0551-65418671 +8618949823763 |
sales@tnjchem.com | China | 34563 | 58 |
| ANHUI WITOP BIOTECH CO., LTD | +8615255079626 |
eric@witopchemical.com | China | 23541 | 58 |
| Dayang Chem (Hangzhou) Co.,Ltd. | 571-88938639 +8617705817739 |
info@dycnchem.com | China | 52849 | 58 |
| changzhou huayang technology co., ltd | +8615250961469 |
2571773637@qq.com | China | 9725 | 58 |