Geneticin
![Geneticin Structure](CAS/GIF/108321-42-2.gif)
- CAS No.
- 108321-42-2
- Chemical Name:
- Geneticin
- Synonyms
- G 418;620u/Mg;USPGrade;GENETICIN;418 DisuL;G418 DisuL;G418 SULFATE;G418 SOLUTION;G-418 SULPHATE;G-418,Geneticin
- CBNumber:
- CB5852743
- Molecular Formula:
- C20H42N4O14S
- Molecular Weight:
- 594.63
- MOL File:
- 108321-42-2.mol
- Modify Date:
- 2023/9/26 17:18:59
SAFETY
Risk and Safety Statements
Symbol(GHS) | ![]() GHS08 |
---|---|
Signal word | Danger |
Hazard statements | H317-H334 |
Precautionary statements | P261-P272-P280-P284-P302+P352-P333+P313 |
Hazard Codes | Xn,Xi |
Risk Statements | 20/21/22-36/37/38-42/43 |
Safety Statements | 22-24/25-36/37-23-36-26-45 |
WGK Germany | 3 |
RTECS | CB9378500 |
HS Code | 29419090 |
Geneticin price More Price(20)
Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
---|---|---|---|---|---|---|---|
Sigma-Aldrich(India) | G8168 | G 418 disulfate salt solution 50?mg/mL in H2O, 0.1 μm filtered, BioReagent, suitable for cell culture | 108321-42-2 | 10ML | ₹12156.48 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | G8168 | G 418 disulfate salt solution 50?mg/mL in H2O, 0.1 μm filtered, BioReagent, suitable for cell culture | 108321-42-2 | 50ML | ₹41297.38 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | G8168 | G 418 disulfate salt solution 50?mg/mL in H2O, 0.1 μm filtered, BioReagent, suitable for cell culture | 108321-42-2 | 100ML | ₹59201.93 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | G5013 | G 418 disulfate salt potency: ≥720?μg per mg (dried basis) | 108321-42-2 | 250MG | ₹2662.95 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | G5013 | G 418 disulfate salt potency: ≥720?μg per mg (dried basis) | 108321-42-2 | 1G | ₹8367.73 | 2022-06-14 | Buy |
Geneticin Chemical Properties,Uses,Production
Description
Geneticin (also known as G418, G418 sulfate) is an aminoglycoside antibiotic similar in structure to gentamicin B1, produced by Micromonospora rhodorangea. Geneticin blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells and is commonly used in laboratory research to select genetically engineered cells. Resistance to Geneticin is conferred by the neo gene from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APH 3'II.
Chemical Properties
white to off-white powder
Uses
Geneticin is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL. It is also used to membrane-permeant allosteric effector of hemoglobin therapeutic in cardiovascular diseases and cancer.
Application
Geneticin's most common application is in molecular biology as a selection agent. Geneticin(G418 sulfate) is toxic to bacteria, yeast, protozoa, helminths, and mammalian cells. Resistance is conferred by one of two dominant genes of bacterial origin which can be expressed in eukaryotic cells. G418 is water soluble and can be stored at room temperature for as long as 1 year. Aqueous solutions should be stored frozen. The amount of G418 required for selection will vary with each cell type and growth cycle. Although cells that are multiplying will be affected sooner than those that are not, cells that are in log phase will still require 3 to 7 days for selection. In general, the amount of G418 required for selection of mammalian cells is 400 mg/ml and 200 mg/ml for maintenance.
General Description
The optimal concentration of G418 for selection of resistance will vary according to the organism and/or cell type under investigation. In general, the concentration of active drug required for selection is as follows: Dictyostelium sp.: 10-100 μg/ml Plant cells: 10-100 μg/ml Yeast cells: 0.5-1.0 μg/ml Mammalian cells: 0.1-2.0 μg/ml
Mode of action
Geneticin works by blocking polypeptide synthesis by preventing the elongation step within eukaryotic cells.
Resistance: Resistance to Geneticin is conferred through the NPTll gene in plant cells and the NEO gene in mammalian cells, which encodes aminoglycoside 3'-phosphotransferase.
Action: Geneticin functions by causing a chain of nonsense mutations during translation, ultimately resulting in inhibition of polypeptide synthesis and protein elongation.
Geneticin Preparation Products And Raw materials
Raw materials
Preparation Products
Supplier | Tel | Country | ProdList | Advantage | Inquiry |
---|---|---|---|---|---|
A.J Chemicals | 91-9810153283 | New Delhi, India | 6124 | 58 | Inquiry |
CLEARSYNTH LABS LTD. | +91-22-45045900 | Hyderabad, India | 6351 | 58 | Inquiry |
Pharmaffiliates Analytics and Synthetics P. Ltd | +91-172-5066494 | Haryana, India | 6773 | 58 | Inquiry |
Triveni chemicals | 08048762458 | New Delhi, India | 6093 | 58 | Inquiry |
Maxcell Systems Pvt. Ltd. | 08046046154 | Karnataka, India | 8 | 58 | Inquiry |
Ayonex Labs Private Limited | 08045911767 | Punjab, India | 12 | 58 | Inquiry |
Pharma Affiliates | 172-5066494 | Haryana, India | 6761 | 58 | Inquiry |
TCI Chemicals (India) Pvt. Ltd. | 1800 425 7889 | New Delhi, India | 6778 | 58 | Inquiry |
Sisco Research Laboratories Pvt. Ltd. | +91-22-4268 5800 | Mumbai, India | 4317 | 58 | Inquiry |
Sisco Resech Laboratories Private Limited | +91-22-4268 5800 | Mumbai, India | 655 | 58 | Inquiry |
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- Use and Mechanism of Geneticin(G418)
- G418 (Geneticin) is an aminoglycoside antibiotic similar in structure to gentamicin B1. It is produced by Micromonospora rhodo....
- Aug 18,2022