GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
![GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE Structure](CAS/GIF/9001-50-7.gif)
- CAS No.
- 9001-50-7
- Chemical Name:
- GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE
- Synonyms
- gapd;G3PD;cb609;GAPDH;G-3-PDH;MGC88685;mg:bb02e05;EC: 1.2.1.12;EC 1.2.1.1.2;GAPDH)/CH8530
- CBNumber:
- CB6673129
- Molecular Formula:
- C3H7O6P
- Molecular Weight:
- 170.057841
- MOL File:
- 9001-50-7.mol
- Modify Date:
- 2023/4/23 13:52:06
storage temp. | -20°C |
---|---|
form | suspension |
color | white |
EPA Substance Registry System | Dehydrogenase, glyceraldehyde phosphate (9001-50-7) |
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE price More Price(7)
Manufacturer | Product number | Product description | CAS number | Packaging | Price | Updated | Buy |
---|---|---|---|---|---|---|---|
Sigma-Aldrich(India) | G2267 | Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle lyophilized powder, ≥75?units/mg protein | 9001-50-7 | 500UNITS | ₹6748.8 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | SRE0024 | Glyceraldehyde-3-phosphate dehydrogenase human recombinant, expressed in Escherichia coli | 9001-50-7 | 100μG | ₹43056.9 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | G2267 | Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle lyophilized powder, ≥75?units/mg protein | 9001-50-7 | 1000UNITS | ₹11199.9 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | G6019 | Glyceraldehyde-3-phosphate Dehydrogenase from human erythrocytes lyophilized powder, 50-150?units/mg protein | 9001-50-7 | 100UNITS | ₹39482.7 | 2022-06-14 | Buy |
Sigma-Aldrich(India) | G5262 | GAPDH standard for protein electrophoresis | 9001-50-7 | 1VIAL | ₹6027.3 | 2022-06-14 | Buy |
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE Chemical Properties,Uses,Production
Uses
Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used:
- for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay
- to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice
- in anti-aggregation assays
- in purified GAPDH studies
General Description
GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway. GAPDH has also been found to function in additional cellular process, such as transcription, apoptosis, oxidative stress and ER to Golgi transport.
Purification Methods
Purify the dehydrogenase from rabbit muscle by extraction with 0.03N KOH and precipitate it with (NH4)2SO4 (0.52 of saturation). The clear supernatant is adjusted to pH 7.5, and NH3 is added dropwise to pH 8.2-8.4. Crystals appear sometimes even without seeding. The crystals are dissolved in H2O, filtered to remove suspended material and 2 volumes of saturated (NH4)2SO4 at pH 8.2-8.4 is added. After 1hour the crystals appear. Recrystallise it in the same way. [Cori et al. J Biol Chem 173 605 1948, Furfine & Velick J Biol Chem 240 844 1965, The Enzymes 7 243 1963, Lui & Huskey Biochemistry 31 6998 1992.] The Km values are: NADH (3.3WM) and 1,3-diphosphoglycerate (8x10-7M) in pH 7.4 imidazole buffer at 26o, NAD (13WM), glyceraldehyde-3-P (90WM), Pi (2.9x10-4M), and arsenate (69WM) in 8.6 M NaHCO3 buffer at 26oC. [Orsi & Cleland Biochemistry 11 102 1972.]
GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE Preparation Products And Raw materials
Raw materials
Preparation Products
Supplier | Tel | Country | ProdList | Advantage | Inquiry |
---|---|---|---|---|---|
career henan chemical co | +86-0371-86658258 +8613203830695 | China | 29898 | 58 | Inquiry |
Chongqing Chemdad Co., Ltd | +86-023-6139-8061 +86-86-13650506873 | China | 39916 | 58 | Inquiry |
Dideu Industries Group Limited | +86-29-89586680 +86-15129568250 | China | 25196 | 58 | Inquiry |
Hebei Guanlang Biotechnology Co., Ltd. | +86-19930503259 +86-19930503259 | China | 18435 | 58 | Inquiry |
Shanghai Acmec Biochemical Technology Co., Ltd. | +undefined18621343501 | China | 33350 | 58 | Inquiry |
Wuhan Fortuna Chemical Co.,Ltd | +8618007136271 | China | 5639 | 58 | Inquiry |
Watson Biotechnology Co.,Ltd | +86-18186686046 +86-18186686046 | China | 5821 | 58 | Inquiry |
Lingyuan Bebiansen Biological Technology Co., Ltd | 18642120371 | China | 7994 | 58 | Inquiry |
Hubei widely chemical technology Co., Ltd. | 027-83778895 18602735115 | China | 2000 | 64 | Inquiry |
Shanghai Zheyan Biotechnology Co., LTD | 18017610038 18017610038 | China | 5000 | 58 | Inquiry |
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