2′-デオキシグアノシン 一水和物 化学特性,用途語,生産方法
外観
白色, 結晶〜粉末
溶解性
水に溶けにくい(0.1g/100mL, 加温)。ほとんどの有機溶媒に不溶、酸で加水分解を受けやすい。DMSO, メチルセロソルブに微溶、水に難溶。
用途
核酸・遺伝子研究用、核酸合成用試薬。
説明
2''-Deoxyguanosine is a purine nucleoside with diverse biological activities. It inhibits the clonogenic growth of HL-60 and K562 leukemia cells (IC
50s = 80 and 100 μM, respectively). 2''-Deoxyguanosine inhibits the growth of MOLT-4 T cells and MGL-8 B cells by 99.8 and 68.3%, respectively, when used at a concentration of 50 μM. It increases the number of binucleated cells, a marker of inhibited cytokinesis, in
A. sativum meristems. 2''-Deoxyguanosine (>1 μM) also induces relaxation of precontracted isolated bovine lingual artery.
化学的特性
White to Off-White Solid. Soluble in NH4OH 1 M: 50 mg/mL, clear to very faintly turbid.
定義
ChEBI: 2'-Deoxyguanosine monohydrate is a purine 2'-deoxyribonucleoside having guanine as the nucleobase. It has a role as a Saccharomyces cerevisiae metabolite, a human metabolite, an Escherichia coli metabolite and a mouse metabolite. It is a purines 2'-deoxy-D-ribonucleoside and a purine 2'-deoxyribonucleoside. It is functionally related to a guanosine.
主な応用
2′-Deoxyguanosine monohydrate has been used as a nucleoside supplement:
to study its effect on mitochondrial DNA copy number in deoxyguanosine kinase (dguok) mutant zebrafish,
in tissue culture medium for deoxyribonucleotide triphosphate (dNTP) synthesis,
in RPMI (Roswell Park Memorial Institute)-1640 medium to eliminate the endogenous thymocytes.
生物学の機能
lu et al. found that 2'-Deoxyguanosine (2-dG) is a key immune signaling molecule in plant–microbe interactions and that Arabidopsis VENOSA4 (VEN4) is essential for plant immunity. 2-dG is the immunoreactive component in ZhiNengCong (ZNC). This compound induced classical immune responses, including ROS production, allose accumulation and MPKs phosphorylation, and upregulated the expression of plant immunity-associated genes. In addition, 2-dG-induced plant immunity requires salicylic acid (SA) receptor NPR1 (but not SA), ethylene (ET) signaling, pattern-recognition receptors/coreceptors (PRRs/coreceptors) and the ATP receptor P2K1[1]. VEN4 was involved in 2-dG biosynthesis and could convert dGTP to 2-dG.
2′-デオキシグアノシン 一水和物 上流と下流の製品情報
原材料
準備製品