아이소프로필 베타-D-싸이오갈락토피라노시드

아이소프로필 베타-D-싸이오갈락토피라노시드 속성

아이소프로필 베타-D-싸이오갈락토피라노시드 MSDS

IPTG

아이소프로필 베타-D-싸이오갈락토피라노시드 C화학적 특성, 용도, 생산

개요

IPTG (also known as Isopropyl-β-D-Thiogalactopyranoside) is a molecular biology reagent that functions as an inducer of galactosidase activity by binding to and inhibiting the repressor. It is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. IPTG cannot be hydrolyzed or broken down by the E. coli therefore the concentration remains constant during cell replication. IPTG is often used at a final concentration ranging from 0.5-1.0 mM. X-Gal is often used with IPTG since it provides an easy visual method to monitor whether protein expression has occurred. X-Gal as the name implies contains a galactose group that once metabolized causes a bright blue color change.

화학적 성질

White crystalline powder

용도

Isopropyl-β-D-thiogalactoside is used as a reagent in molecular biology. It is used as an effective β-galactosidase inducer of protein expression where the gene is under the control of the lac operator. It is used with 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside to identify the non-recombinant plasmid in cloning experiments by blue-white screen methodology.

제조 방법

a synthetic method of isopropyl-β-D-thiogalactoside. The steps are as follows:
Step 1: Dissolve β-D-galactose pentaacetate in any organic solvent of methylene chloride, chloroform or 1,2-dichloroethane, and add Lewis acid (boron trifluoride ether, (Aluminum trichloride, zinc chloride) catalyst and potassium ethylxanthate or sodium ethylxanthate are reacted to obtain tetraacetylgalactose ethylxanthate after treatment.
Step 2: Dissolve tetraacetyl galactose ethyl xanthate, 2-bromopropane and sodium carbonate in methanol or ethanol at a temperature of 20°C to 80°C and react for 4-6 hours to obtain isopropyl group after treatment Isopropyl-β-D-thiogalactoside.

정의

ChEBI: Isopropyl beta-D-thiogalactopyranoside is an S-glycosyl compound consisting of beta-D-1-thiogalactose having an isopropyl group attached to the anomeric sulfur.

주요 응용

IPTG (Isopropyl-β-D-thiogalactopyranoside) is a galactose analogue not recognized by β-galactosidase. IPTG is a non-metabolizable galactose analog that induces expression of the lac operon in Escherichia coli. It is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of β-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

일반 설명

Inducer for β-galactosidase, an enzyme that promotes lactose utilization. Use in conjunction with X-Gal. Molecular Biology grade.
Stock solutions:
X-Gal: 20 mg/ml in DMF
IPTG: 20 mg/ml in water
Use 4:1 (X-Gal:IPTG)
Assay: ≥99%
Dioxane: None detected
Melting point: 110-114°C
Specific rotation [a]25/D (1%, water): -34.0 to -29.0°
Intended for laboratory and manufacturing use only. Not for drug, food, or household use.

Solubility in organics

IPTG is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF). The solubility of IPTG in ethanol and DMSO is approximately 20 mg/ml and approximately 30 mg/ml in DMF.

아이소프로필 베타-D-싸이오갈락토피라노시드 준비 용품 및 원자재

원자재

준비 용품

아이소프로필 베타-D-싸이오갈락토피라노시드 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Hubei Yiling biotechnology Co Ltd	+86-18163349544 +86-18163349544	mollylucking@foxmail.com	China	663	58
Siyang Liaoning Biochemical Products Co. Ltd	+86-15221114306; +8615221114306	2594582574@qq.com	China	561	58
XuZhou Magic Biotechnology Co., Ltd.	+undefined13921770081	SVP01@magicbiotech.cn	China	92	58
Jiangsu Boquan Biotechnology Co., Ltd.	+86-18168774353	boquanshengwu003@boquansw.com	China	248	58
Wuhan BJM Pharm Inc	+86-027-5234-1919 +8613545198479	sales03@benjaminpharmchem.com	China	690	58
Hebei Andu Technology Com.,Ltd	+86-86-17798073498 +8617798073498	admin@hbandu.com	China	299	58
Xinxiang Aurora Biotechnology Co.,Ltd.	+86-0086-0373-3088722 +8618637352520	info@aurora-biotech.com	China	93	58
Hebei Weibang Biotechnology Co., Ltd	+8615531157085	abby@weibangbio.com	China	8810	58
Hebei Chuanghai Biotechnology Co,.LTD	+86-13131129325	sales1@chuanghaibio.com	China	5892	58
Hebei Kingfiner Technology Development Co.Ltd	+86-15532196582 +86-15373005021	lisa@kingfinertech.com	China	3010	58

아이소프로필 베타-D-싸이오갈락토피라노시드 관련 검색: