PCR NUCLEOTIDE MIX

PCR NUCLEOTIDE MIX 구조식 이미지
카스 번호:
상품명:
PCR NUCLEOTIDE MIX
동의어(영문):
DNTP/DUTP MIX;PCR NUCLEOTIDE MIX;PCR NUCLEOTIDE MIXPLUS;ADVANTAGE PCR DEOXYNUCLEOTIDE MIX
CBNumber:
CB2373124
분자식:
포뮬러 무게:
0
MOL 파일:
Mol file

PCR NUCLEOTIDE MIX 속성

저장 조건
-20°C
물리적 상태
액체

안전

PCR NUCLEOTIDE MIX C화학적 특성, 용도, 생산

용도

  • This ready-to-use nucleotide mix is a premixed solution of the sodium salts of dATP, dGTP, and dCTP, each at a concentration of 10 mM, and dUTP at a concentration of 30 mM in water. This mix is optimized for use in all types of amplification reactions: PCR
  • RT-PCR
  • Prevention of carryover contamination
To allow decontamination of PCR or RT-PCR, dUTP in place of dTTP is incorporated into the PCR product. Subsequent reactions may then be treated with Uracil-DNA Glycosylase (UNG). Avoiding the need of re-opening the reaction vial, the vials are incubated at +20 °C, resulting in the degradation of potentially contaminating uracil-containing amplification products. During this step, template DNA and RNA remain unaffected, since normal DNA does not contain uracil, and RNA does not serve as a substrate for UNG. Before starting the actual thermocycling program, UNG is inactivated by incubation at +95 °C. Uracil -DNA Glycosylase, heat-labile is particularily useful, as it is fully inactivated already after incubation at +95 °C for 2 minutes. The natural enzyme from E. coli requires incubating the reaction mixture for 10 minutes at +95 °C. The shorter heat treatment substantially reduces the risk for loosing the template nucleic acid, which typically is present at low concentrations only. This is of particular importance, when performing RT-PCR. We therefore recommend the use of Uracil-DNA Glycosylase.
It has been used in LightCycler PCR assay to identify B. pertussis and B.parapertussis in nasopharyngeal swabs. It has also been used in quantitative PCR (qPCR) assay.

일반 설명

PCR Nucleotide MixPLUS is a clear, colorless solution of the sodium salts of dATP, dCTP, dGTP, each at a concentration of 10 mM, and dUTP at a concentration of 30 mM in Water, PCR Grade. This nucleotide mixture can be added directly to polymerase chain reactions.
The incorporation of dUTP in place of dTTP allows the degradation of contaminating PCR products from former reactions with Uracil-DNA Glycosylase (UNG) to prevent carryover contamination from previous amplifications.

PCR NUCLEOTIDE MIX 준비 용품 및 원자재

원자재

준비 용품


PCR NUCLEOTIDE MIX 공급 업체

글로벌( 12)공급 업체
공급자 전화 이메일 국가 제품 수 이점
Sigma-Aldrich 021-61415566 800-8193336
orderCN@merckgroup.com China 51471 80

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