아크리딘오렌지
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아크리딘오렌지 속성
- 녹는점
- 165 °C (dec.)(lit.)
- 끓는 점
- 469℃
- 밀도
- 1.169
- 굴절률
- 1.6080 (estimate)
- 인화점
- 237℃
- 저장 조건
- Sealed in dry,Room Temperature
- 용해도
- DMF: 2 mg/ml; DMSO: 20 mg/ml; Ethanol: 0.3 mg/ml; PBS (pH 7.2): 1 mg/ml
- 물리적 상태
- 결정성 고체
- 색상 색인 번호
- 46005
- 산도 계수 (pKa)
- 9.97±0.30(Predicted)
- 색상
- 에탄올에서 결정화 된 노란색 바늘 모양의 물질
- 안정성
- 감광성
- IARC
- 3 (Vol. 16, Sup 7) 1987
안전
안전지침서 | 22-24/25 | ||
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WGK 독일 | 3 | ||
RTECS 번호 | AR7600000 | ||
독성 | mmo-omi 10 mg/L MIBLAO 49,223,80 | ||
기존화학 물질 | KE-03041 |
아크리딘오렌지 C화학적 특성, 용도, 생산
물성
분자 내 아크리딘 고리를 가진 염기성 염료를 말한다. 물이나 에탄올에 녹는 분말인데, 이것의 에탄올 용액은 황록색의 형광을 낸다.용도
주로 가죽 염료로 많이 사용되는데, 후 처리시 제대로 정화되지 않는 폐액 때문에 2차 오염의 피해 가능성이 있다.개요
Acridine orange is a cell-permeable, nucleic acid-selective fluorescent cationic dye useful for cell cycle determination and detection of cellular autophagy. It exhibits excitation/emission spectra of 502/525, 460/650, and 475/590 nm when bound to dsDNA, bound to ssDNA or RNA, and under acidic conditions, respectively. The ratio of acridine orange emission at 590 to emission at 525 nm can be used to quantify the increase in the number of acidic vesicular organelles observed during cellular autophagy.정의
ChEBI: A member of the class of aminoacridines that is acridine carrying two dimethylamino substituents at positions 3 and 6. The hydrochloride salt is the fluorescent dye 'acridine orange', used for cell cycle determination.제조 방법
4,4′-Bis(N,N-dimethyl)aminodiphenylmethane?dinitration, the resultant 4-(4-(Dimethylamino)-3-nitrobenzyl)-N,N-dimethyl-2-nitrobenzenamine?reduction, and acid heating will diamino compound cyclization, then will product oxidation and made free base.Safety Profile
Poison by subcutaneous route.Questionable carcinogen with experimental tumorigenicand carcinogenic data. Mutation data reported. Whenheated to decomposition it emits toxic fumes of NOx.Purification Methods
The double salt with ZnCl2 (6g) is dissolved in water (200mL) and stirred with four successive portions (12g each) of Dowex-50 ion-exchange resin (K+ form) to remove the zinc. The solution is then concentrated in vacuum to 20mL, and 100mL of ethanol is added to precipitate KCl which is removed. Ether (160mL) is added to the solution from which, on chilling, the dye crystallises as its chloride. It is separated by centrifugation, washed with chilled ethanol and ether, and dried under vacuum, before being recrystallised from ethanol (100mL) by adding ether (50mL), and chilling. Yield 1g. [Pal & Schubert J Am Chem Soc 84 4384 1962]. It was recrystallised twice as the free base from ethanol or methanol/water by dropwise addition of NaOH (less than 0.1M). The precipitate was washed with water and dried under vacuum. It was dissolved in CHCl3 and chromatographed on alumina: the main sharp band was collected, concentrated and cooled to -20o. The precipitate was filtered off, dried in air, then dried for 2hours under vacuum at 70o. [Stone & Bradley J Am Chem Soc 83 3627 1961, Blauer & Linschitz J Phys Chem 66 453 1962, Albert J Chem Soc 244 1947, Beilstein 22 III/IV 5490, 22/11 V 326.]아크리딘오렌지 준비 용품 및 원자재
원자재
준비 용품
아크리딘오렌지 공급 업체
글로벌( 103)공급 업체
공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
---|---|---|---|---|---|
Shaanxi Dideu Medichem Co. Ltd | 18192627656 |
1012@dideu.com | China | 2470 | 58 |
Hebei Mojin Biotechnology Co., Ltd | +8613288715578 |
sales@hbmojin.com | China | 12456 | 58 |
Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 |
info@tianfuchem.com | China | 21669 | 55 |
Hebei Guanlang Biotechnology Co., Ltd. | +86-19930503282 |
alice@crovellbio.com | China | 8822 | 58 |
career henan chemical co | +86-0371-86658258 +8613203830695 |
factory@coreychem.com | China | 29826 | 58 |
Antai Fine Chemical Technology Co.,Limited | 18503026267 |
info@antaichem.com | CHINA | 9641 | 58 |
Career Henan Chemica Co | +86-0371-86658258 +8613203830695 |
laboratory@coreychem.com | China | 30253 | 58 |
Hefei TNJ Chemical Industry Co.,Ltd. | 0551-65418671 |
sales@tnjchem.com | China | 34571 | 58 |
Henan Alfa Chemical Co., Ltd | +8618339805032 |
alfa4@alfachem.cn | China | 13187 | 58 |
Guangzhou TongYi biochemistry technology Co.,LTD | +8613073028829 |
mack@tongyon.com | China | 2996 | 58 |
아크리딘오렌지 관련 검색:
p-[[2-methyl-4-[(o-tolyl)azo]phenyl]azo]phenol
4',5',7-Tribromo-3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dicarboxylic acid
SOLVENT ORANGE 22
Solvent Orange 14
4',5'-DINITROFLUORESCEIN
D&CORANGENO.16
AZOSOL FAST ORANGE RA
SOLVENT ORANGE 23
Solvent Orange 2
10-OCTADECYLACRIDINE ORANGE BROMIDE
ACRIDINE ORANGE
2,8-BIS(DIMETHYLAMINO)-10-DODECYL-ACRIDINIUM BROMIDE
NONYL ACRIDINE ORANGE
Basic Orange 14
ACRIDINE ORANGE