역전사효소,M-MLV,RNASEH+
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역전사효소,M-MLV,RNASEH+ 속성
- 저장 조건
- -20°C
- 물리적 상태
- 액체
- 색상
- 무색의
안전
WGK 독일 | 3 | ||
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HS 번호 | 3504009000 |
역전사효소,M-MLV,RNASEH+ C화학적 특성, 용도, 생산
개요
Enzyme possessed by retroviruses that allows transcription of DNA from an RNA template. Such an enzyme has never been isolated from other sources, but speculation about its possible occurrence continues. Such an enzyme is widely used experimentally in genetic engineering to allow the formation of complementary DNA (cDNA) from purified RNA. The discovery of this enzyme faulted the widely held central dogma, which stated that the information flow in nature was always from DNA to RNA.용도
Synthesizes a complementary DNA strand using single-stranded RNA or DNA in the presence of a primer일반 설명
M-MLV (Moloney murine leukemia virus) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.Purification Methods
This enzyme produces the complementary DNA from the RNA (as template). These are purified by solubilising the virus with non-ionic detergents. Lysed virions are adsorbed on DEAE-cellulose or DEAE-Sephadex columns, and the enzymes are eluted with a salt gradient, then chromatographed on a phosphocellulose column and fractions with enzyme activity are eluted in a salt gradient. They are also purified from other viral proteins by affinity chromatography on a pyran-Sepharose column. [Verna Biochim Biophys Acta 473 1 1977, Smith Methods Enzymol 65 560 1980; see commercial catalogues for other transcriptases.]역전사효소,M-MLV,RNASEH+ 준비 용품 및 원자재
원자재
준비 용품
역전사효소,M-MLV,RNASEH+ 공급 업체
글로벌( 132)공급 업체
공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
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Shanghai Daken Advanced Materials Co.,Ltd | +86-371-66670886 |
info@dakenam.com | China | 16911 | 58 |
career henan chemical co | +86-0371-86658258 +8613203830695 |
factory@coreychem.com | China | 29825 | 58 |
Creative Enzymes | 1-516-855-7709 |
info@creative-enzymes.com | United States | 8748 | 58 |
Hebei Fengjia New Material Co., Ltd | +86-0311-87836622 +86-17333973358 |
sales06@hbduling.cn | China | 8078 | 58 |
Nanjing Duly Biotech Co.,Ltd | +undefined18013301590 |
melody@njduly.com | China | 2452 | 58 |
Watson Biotechnology Co.,Ltd | +86-18186686046 +86-18186686046 |
sales01@watsonbiotech.cn | China | 5855 | 58 |
J & K SCIENTIFIC LTD. | 010-82848833 400-666-7788 |
jkinfo@jkchemical.com | China | 96815 | 76 |
BEST-REAGENT | 400-1166-196 18981987031 |
cdhxsj@163.com | China | 11726 | 57 |
Chengdu Ai Keda Chemical Technology Co., Ltd. | 4008-755-333 18080918076 |
800078821@qq.com | China | 9725 | 55 |
Shanghai Aladdin Bio-Chem Technology Co.,LTD | 400-400-6206333 18521732826 |
market@aladdin-e.com | China | 25013 | 65 |
역전사효소,M-MLV,RNASEH+ 관련 검색:
T4 DNA 폴리머라제 디스프로슘 트리(2,2,6,6-테트라메틸-3,5-헵탄디오네이트) 아세틸아세톤산 구리 아세틸아세토산 제2철 실버 아세틸아세토네이트 알루미늄 아세틸아세토네이트 코발트(II) 아세틸아세토네이트 하이드레이트
RNA-directed RNA polymerase
DICHLORO(ETHYLENEDIAMINE)PLATINUM(II)
TERT-BUTYL ISOCYANIDE
Tosylmethyl isocyanide
N-BUTYLISOCYANIDE
Benzyl isocyanide
Ethyl isocyanoacetate
PHENYLSELENOL
Tris(2,4-pentanedionato)chroMiuM(III)
TRIS(2,2,6,6-TETRAMETHYL-3,5-HEPTANEDIONATO)EUROPIUM(III)
1,1,3,3-TETRAMETHYLBUTYL ISOCYANIDE