Digoxigenin-11-dUTP, alkali-stable
|
|
Digoxigenin-11-dUTP, alkali-stable 속성
- 저장 조건
- -20°C
- 물리적 상태
- 액체
안전
Digoxigenin-11-dUTP, alkali-stable C화학적 특성, 용도, 생산
용도
Digoxigenin-11-dUTP, alkali-stable has been used to label probes forfluorescent in situ hHybridization (FISH) and polymerase chain reaction (PCR)
Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will remain attached. It is therefore ideal for use in DIG-labeled DNA that needs to survive exposure to alkaline treatment. However, if you intend to make a DIG-labeled probe that can be removed by alkali denaturation (e.g ., during stripping and reprobing of blots), do not use this alkali-stable preparation. Instead, use the alkali-labile form of DIG-11-dUTP.
일반 설명
Digoxigenin-11-dUTP, alkali-stable is provided in 1 mM tetralithium salt, solution.Biochem/physiol Actions
Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphosphate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. DIG-11-dUTP can also replace dTTP in labeling during polymerase chain reaction (PCR) (e.g., with Taq DNA Polymerase), 3′-tailing (with terminal transferase) and cDNA synthesis (e.g., with transcriptor or M-MuLV reverse transcriptase). It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.Digoxigenin-11-dUTP, alkali-stable 준비 용품 및 원자재
원자재
준비 용품
Digoxigenin-11-dUTP, alkali-stable 공급 업체
글로벌( 1)공급 업체
공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
---|---|---|---|---|---|
Sigma-Aldrich | 021-61415566 800-8193336 |
orderCN@merckgroup.com | China | 51471 | 80 |