ANTI-GOLGI BETA-COP PROTEIN

ANTI-GOLGI BETA-COP PROTEIN C화학적 특성, 용도, 생산

용도

Monoclonal Anti-β-COP antibody produced in mouse is suitable for use as a primary antibody in immunoblot:

• analysis at a working dilution of 1:1000 using subcellular proteins from rat PC12 (pheochromocytoma) cells
• analysis of gradient fractions of cerebral microvessels to confirm the separation of plasma membrane lipid raft domains from intracellular membranous components
• detection of the Golgi marker protein β-COP in exosome-enriched extracellular microvesicles (eMV) preparations from untreated HeLa cells

It is suitable for immunostaining of β-coatomer, that is used as a intracellular, Golgi protein marker :

• to confirm that CD14 staining is localized to the cell surface of HAEC
• for examining the localization of Meltrin β in the Golgi apparatus and its vicinity in neurons prepared from developing dorsal root ganglia of mouse embryos
• in NB4 and NB4-LR1 cells to examine the colocalization of PKA regulatory subunits
• in CHO cells to examine colocalization of GFP-Rab24

It is suitable for use in cell-surface ELISA of human aortic endothelial cells (HAEC)

It is also suitable for western blot analysis at a working dilution of 1:1000 using a preparation of stacked Golgi membranes from rat liver, for indirect immunofluorescence at a working dilution of 1:80 using cultured Chinese hamster ovary (CHO) cells and for microarray.

일반 설명

The coatomer (approx. 550kDa) consists of proteins designated α-, β-, γ-, and δ-COP, together with substoichiometric amounts of several other proteins.

Biochem/physiol Actions

COPs (coatomer proteins) contain adaptin-like, complex (8) and are transiently attached to the vesicles involved in transport within the Golgi complex and possibly between the rough ER and Golgi complex. β-COP has a molar mass of 110kDa and its primary structure is homologous to the β-adaptin component of clathrin-coated vesicles.

ANTI-GOLGI BETA-COP PROTEIN 준비 용품 및 원자재

원자재

준비 용품

ANTI-GOLGI BETA-COP PROTEIN 공급 업체

공급자	전화	이메일	국가	제품 수	이점
Riedel-de Haen AG	--		United States	6825	87
SIGMA-RBI	--		Switzerland	6913	91
ABR-Affinity BioReagents	--	affinity@bioreagents.com	United States	489	73
Alexis Corporation	--	alexis-ch@alexis-corp.ch	Switzerland	3599	74
Kanto Chemical Co., Inc.	--	reag-info@gms.kanto.co.jp	Japan	6762	74
EMD Biosciences, Inc.	--	technical@calbiochem.com	United States	6529	66
VWR International	--	solutions@vwr.com	United Kingdom	6554	82