디모그 C화학적 특성, 용도, 생산
개요
The pro-
angiogenic factor HIF-
1α is targeted for destruction in normoxic environments by the hydroxylation of a specific proline residue, P564, by the oxygen-
sensing enzyme HIF-
1α prolyl hydroxylase (HIF-
PH). DMOG is a cell permeable, competitive inhibitor of HIF-
PH. It acts to stabilize HIF-
1α expression at normal oxygen tensions in cultured cells, at concentrations between 0.1 and 1 mM. DMOG is therefore expected to act as a pro-
angiogenic compound, acting
via the HIF-
1α system.
화학적 성질
Off-White to Pale Pink Solid
용도
DMOG is a cell permeable prolyl-4-hydroxylase inhibitor which upregulates HIF activity. HIF activation stimulates angiogenesis in several different models (1nM). DMOG also inhibits FIH (factor inhibiting HIF), an asparaginyl hydroxylase, which enhances the HIF response. It is active in vivo and attenuates myocardial injury in a rabbit ischemia reperfusion model (20mg/Kg).
효소 저해제
This iron-binding a-ketoglutarate (2-oxoglutarate) analogue (FW = 147.09
g/mol; also named N-oxaloglycine) competitively inhibits prolyl-4-
hydroxylase (Reaction: Procollagen (L-proline) + a-ketoglutarate + O2 →
Procollagen ( (2S,4R) -4-hydroxyproline) + succinate + CO2). During
catalysis, prolyl-4-hydroxylase forms Fe (III), and the latter most likely
makes an extremely stable metal ion complex with N-oxaloglycine.
Substitution on the glycine moiety alters inhibitor activity stereoselectively
and that, if the w-carboxylate is homologated or replaced, either by
acylsulfonamides or anilide, activity is likewise sharply reduced. Prolyl-
4-Hydroxylase Catalysis: Each catalytic round of this posttranslational
modifying enzyme reaction occurs in two stages. O2 is bound end-on in an
axial position, producing a dioxygen unit. Nucleophilic attack at C-2
generates a tetrahedral intermediate, with loss of the double bond in
dioxygen and bonds to iron and the a-carbon of a-ketoglutarate.
Elimination of CO2 coincides with formation of the Fe (IV) =O species. The
second stage involves the abstraction of the pro-R hydrogen atom from C-4
of proline, followed by radical combination, yielding hydroxyproline. In
the presence of a-ketoglutarate, enzyme-bound Fe2+ is rapidly converted to
Fe3+, resulting in inactivation of the enzyme Ascorbate is utilized as a
cofactor to reduce Fe (III) back to Fe (II). Cell-Permeable Analogue:
Dimethyloxalylglycine (FW = 175.14 g/mol; CAS 89464-63-1; Symbol:
DMOG, also named N- (methoxyoxoacetyl) -glycine methyl ester) is
metabolicaly demethylated to form N-oxaloglycine upon entry to many
cells.
디모그 준비 용품 및 원자재
원자재
준비 용품
