DIG-High Prime DNA Labeling and Detection Starter Kit I 속성
저장 조건
-20°C
안전
위험 및 안전 성명
위험 및 사전주의 사항 (GHS)
그림문자(GHS):
신호 어:
Danger
유해·위험 문구:
암호
유해·위험 문구
위험 등급
범주
신호 어
그림 문자
P- 코드
H315
피부에 자극을 일으킴
피부부식성 또는 자극성물질
구분 2
경고
P264, P280, P302+P352, P321,P332+P313, P362
H318
눈에 심한 손상을 일으킴
심한 눈 손상 또는 자극성 물질
구분 1
위험
P280, P305+P351+P338, P310
예방조치문구:
P264
취급 후에는 손을 철저히 씻으시오.
P264
취급 후에는 손을 철저히 씻으시오.
P280
보호장갑/보호의/보안경/안면보호구를 착용하시오.
P302+P352
피부에 묻으면 다량의 물로 씻으시오.
P332+P313
피부 자극이 생기면 의학적인 조치· 조언을 구하시오.
DIG-High Prime DNA Labeling and Detection Starter Kit I C화학적 특성, 용도, 생산
용도
DIG-High Prime DNA Labeling and Detection Starter Kit I has been used in a variety of hybridization techniques:
in Southern blots
in northern blots
in dot blots
in colony and plaque hybridizations
for all types of filter hybridization
for single-copy gene detection in total genomic DNA, even from organisms with high complexity, for example, human, barley and wheat
DIG-labeled probes are generated at high yield within one hour or after overnight incubation.
일반 설명
The DIG High Prime DNA Labeling and Detection Starter Kit I uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent color detection by enzyme immunoassay. This kit contains a ready-made blocking solution, combined stock solution of of nitroblue tetrazolium (NBT)/ 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers and reaction buffer, all in one convenient reagent. The sample material can be: DNA fragments of at least 100bp, linearized plasmid, cosmid or λDNA, or supercoiled DNA. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves as a template for the synthesis of labeled DNA and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10ng) with this method. In this method, the complementary DNA strand is synthesized by Klenow polymerase using the 3′-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin, present in the reaction are incorporated into the newly synthesized complementary DNA strand. This is a convenient kit for random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This product is designed as a safer chemical.? The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
DIG-High Prime DNA Labeling and Detection Starter Kit I 준비 용품 및 원자재
원자재
준비 용품
DIG-High Prime DNA Labeling and Detection Starter Kit I 공급 업체