N,N-비스(2 하이드록시에틸)-2-아미노에탄설폰 산
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N,N-비스(2 하이드록시에틸)-2-아미노에탄설폰 산 속성
- 녹는점
- 150-155 °C
- 밀도
- 1.210 (estimate)
- 증기압
- 0.001Pa at 25℃
- 굴절률
- 1.5500 (estimate)
- 저장 조건
- room temp
- 용해도
- H2O: 1 M at 20 °C, 투명, 무색
- 물리적 상태
- 가루
- 색상
- 하얀색
- 수소이온지수(pH)
- 2.5-5.0 (25℃, 1M in H2O)
- 산도 계수 (pKa)
- 7.1(at 25℃) 7.15(at 20℃)
- pH 범위
- 6.4 - 7.8
- 수용성
- 녹는
- 최대 파장(λmax)
- λ: 260 nm Amax: 0.095
λ: 280 nm Amax: 0.080
- BRN
- 1781572
- LogP
- -3.59 at 26℃
- CAS 데이터베이스
- 10191-18-1(CAS DataBase Reference)
안전
- 위험 및 안전 성명
- 위험 및 사전주의 사항 (GHS)
N,N-비스(2 하이드록시에틸)-2-아미노에탄설폰 산 C화학적 특성, 용도, 생산
개요
BES is a zwitterionic buffer that is used in biochemistry and molecular biology research. It is one of the "Good" buffers developed in the 1960's to provide buffers in the pH range of 6.15 - 8.35 for wide applicability to biochemical studies. The pioneering publication by Good and co-workers describes the synthesis of BES and its physical properties.A protocol has been reported for the use of BES buffered saline in the calcium phosphate mediated transfection of eukaryotic cells with plasmid DNA. An investigation has been published on the interaction of BES and other amine buffers with DNA and the measurement of these complexes by free solution capillary electrophoresis.
The effect of BES and other zwitterionic buffers on the bicinchoninic acid (BCA) measurement of microgram quantities of protein has been studied. BES was found to diminish color development of the proteinBCA complex by 30-35% at 50 mM, and by <10% at 5 mM, measuring in the 1-10 mg range of BSA against protein-BCA complexes formed in water. This color loss may result from binding of Cu2+ to BES.
화학적 성질
White/clear cryst. powder용도
BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C. Absorbance (100 mM, 260 nm):≤0.05.BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
주요 응용
BES, or N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, can be used to study molecular biology, biological buffers, molecular biology reagents and zwitterionic compounds. BES has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES has also been used to determine that saline and buffers minimize the action of interfering factors in the bacterial endotoxins test.BES is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.
제조 방법
First, sodium vinyl sulfonate and diethanolamine synthesize N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium by addition reaction. Through the strong acid ion exchange resin sodium ions, the eluent concentrated after adding methanol crystallization. Finally, BES is obtained after precipitation.일반 설명
BES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) is a useful secondary standard biochemical buffer. Useful pH range for BES is 6.4 to 7.8. It is useful for diagnostic assay manufacturing industry. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes.Purification Methods
Crystallise BES from aqueous EtOH. [Beilstein 4 IV 3290.]N,N-비스(2 하이드록시에틸)-2-아미노에탄설폰 산 준비 용품 및 원자재
원자재
준비 용품
N,N-비스(2 하이드록시에틸)-2-아미노에탄설폰 산 공급 업체
글로벌( 405)공급 업체
공급자 | 전화 | 이메일 | 국가 | 제품 수 | 이점 |
---|---|---|---|---|---|
Hebei Mojin Biotechnology Co., Ltd | +86 13288715578 +8613288715578 |
sales@hbmojin.com | China | 12447 | 58 |
Henan Bao Enluo International TradeCo.,LTD | +86-17331933971 +86-17331933971 |
deasea125996@gmail.com | China | 2503 | 58 |
Watson Biotechnology Co.,Ltd | +86-18186686046 +86-18186686046 |
sales01@watsonbiotech.cn | China | 5850 | 58 |
Capot Chemical Co.,Ltd. | 571-85586718 +8613336195806 |
sales@capotchem.com | China | 29798 | 60 |
Henan Tianfu Chemical Co.,Ltd. | +86-0371-55170693 +86-19937530512 |
info@tianfuchem.com | China | 21667 | 55 |
Shanghai Time Chemicals CO., Ltd. | +86-021-57951555 +8617317452075 |
jack.li@time-chemicals.com | China | 1807 | 55 |
career henan chemical co | +86-0371-86658258 +8613203830695 |
sales@coreychem.com | China | 29888 | 58 |
Hubei Jusheng Technology Co.,Ltd. | 18871490254 |
linda@hubeijusheng.com | CHINA | 28180 | 58 |
Shanghai Longyu Biotechnology Co., Ltd. | +8619521488211 |
info@longyupharma.com | China | 2534 | 58 |
Chongqing Chemdad Co., Ltd | +86-023-6139-8061 +86-86-13650506873 |
sales@chemdad.com | China | 39916 | 58 |
N,N-비스(2 하이드록시에틸)-2-아미노에탄설폰 산 관련 검색:
에틸디에탄올아민(노르말) 타우린 다이에탄올아민 N-메틸다이에탄올아민 N,N-비스(2 하이드록시에틸)-2-아미노에탄설폰 산
KLH, BES/Mg2+ Buffer
Dermol BES
2-Diethylaminoethanethiol
HYPOTAURINE
2-(DIMETHYLAMINO)ETHANETHIOL
N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt
N-METHYLTAURINE
METHYLXYLENOL BLUE
BES ,Free acid
Ethanesulfonic acid
BES BUFFER
BES BUFFERED SALINE
BES-1 protein