CU-T12-9
| 中文名称 | CU-T12-9 |
|---|---|
| 中文同义词 | 化合物CU-T12-9;N-甲基-4-硝基-2-(4-(4-(三氟甲基)苯基)-1H-咪唑-1-基)苯胺;CU-T12-9,TLR1 / 2激动剂 |
| 英文名称 | CU-T12-9 |
| 英文同义词 | CU-T12-9;N-methyl-4-nitro-2-[4-[4-(trifluoromethyl)phenyl]imidazol-1-yl]aniline;Benzenamine, N-methyl-4-nitro-2-[4-[4-(trifluoromethyl)phenyl]-1H-imidazol-1-yl]-;obesity,inflammatory,carcinoma,cancer,Toll-like Receptor (TLR),TLR1/2,diseases,specific,infectious,bladder,CU-T-12-9,CUT129,Inhibitor,breast,asthma,CU T12 9,inhibit,influenza,pancreatic |
| CAS号 | 1821387-73-8 |
| 分子式 | C17H13F3N4O2 |
| 分子量 | 362.31 |
| EINECS号 | |
| 相关类别 | |
| Mol文件 | 1821387-73-8.mol |
| 结构式 |  |
CU-T12-9 性质
| 沸点 | 524.3±50.0 °C(Predicted) |
|---|---|
| 密度 | 1.39±0.1 g/cm3(Predicted) |
| 储存条件 | Store at -20°C |
| 溶解度 | 二甲基亚砜：250 mg/mL（690.02 mM） |
| 形态 | 固体 |
| 酸度系数(pKa) | 3.53±0.10(Predicted) |
| 颜色 | 浅黄至黄色 |
|
TLR2 52.9 nM (EC 50 , in HEK-Blue cells) |
TLR1 52.9 nM (EC 50 , in HEK-Blue cells) |
CU-T12-9 directly targets TLR1/2 to initiate downstream signaling. By binding to both TLR1 and TLR2, CU-T12-9 facilitates the TLR1/2 heterodimeric complex formation, which in turn activates the downstream signaling.
CU-T12-9 activates the TLR1/2 pathway by inducing NF-κB activation to trigger downstream signaling, such as secreted embryonic alkaline phosphatase (SEAP), NO, and TNF-α.
CU-T12-9 (0.39-100 μM; 24 hours) does not produce toxicity up to 100 μM in HEK-Blue hTLR2 and Raw 264.7 cells.
CU-T12-9 up-regulates the mRNA levels of TLR1, TLR2, TNF, IL-10, and iNOS. CU-T12-9 (0.1-10 μM) activates TLR1 mRNA and iNOS mRNA after Raw 264.7 cells are treated for 24 hours. CU-T12-9 (0.1-10 μM) activates TLR2 and IL-10 mRNA after Raw 264.7 cells are treated for 2 hours. CU-T12-9 (0.1-10 μM) activates TNF mRNA after Raw 264.7 cells are treated for 8 hours.
Cell Cytotoxicity Assay
| Cell Line: | HEK-Blue hTLR2 and Raw 264.7 macrophage cells |
| Concentration: | 0.39, 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, and 100 μM |
| Incubation Time: | 24 hours |
| Result: | No toxicity was seen up to 100 μM. |
RT-PCR
| Cell Line: | Raw 264.7 cells |
| Concentration: | 0.1, 1, 10 μM |
| Incubation Time: |
24 hours for TLR1 and iNOS mRNA assay
2 hours for TLR2 and IL-10 mRNA assay 8 hours for TNF mRNA assay |
| Result: |
Triggered TLR1 mRNA and iNOS mRNA at 24 hours dose-dependently.
Dose-dependent activation of TLR2 mRNA and IL-10 mRNA at 2 hours. Showed dose-dependent activation of TNF mRNA at 8 hours. |
安全信息
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024/04/30 | HY-110353 | CU-T12-9 | 1 mg | 522元 | |
| 2024/04/30 | HY-110353 | CU-T12-9 CU-T12-9 | 1821387-73-8 | 5mg | 1100元 |