CA-5F
| 中文名称 | CA-5F |
|---|---|
| 中文同义词 | 化合物CA-5F;(E)-3-((1H-吲哚-3-基)亚甲基)-5-((E)-3,4-二甲氧基亚)-1-甲基哌啶-4-酮;(E)-3-((1H-吲哚-3-基)亚甲基)-5-((E)-3,4-二甲氧基亚基)-1-甲基哌啶-4-酮 |
| 英文名称 | CA-5f |
| 英文同义词 | CA-5f;CA-5F;CA 5F;CA5F;1370032-19-1;4-Piperidinone, 3-[(3,4-dimethoxyphenyl)methylene]-5-(1H-indol-3-ylmethylene)-1-methyl-, (3E,5E)-;-5-(1H-indol-3-ylmethylene)-1-methyl-, (3E,5E)-;(E)-3-((1H-Indol-3-yl)methylene)-5-((E)-3,4-dimethoxybenzylidene)-1-methylpiperidin-4-one;CA-5f,Apoptosis,inhibit,Autophagy,Inhibitor,CA 5f,CA5f;(3E,5E)-3-(3,4-dimethoxybenzylidene)-5-[(1H-indol-3-yl)methylene]-1-methylpiperidin-4-one |
| CAS号 | 1370032-19-1 |
| 分子式 | C24H24N2O3 |
| 分子量 | 388.46 |
| EINECS号 | |
| 相关类别 | 对照品;API |
| Mol文件 | 1370032-19-1.mol |
| 结构式 |  |
CA-5F 性质
| 沸点 | 639.7±55.0 °C(Predicted) |
|---|---|
| 密度 | 1.253±0.06 g/cm3(Predicted) |
| 储存条件 | Store at -20°C |
| 溶解度 | DMSO：77.5 mg/mL (199.51 mM)；水：< 0.1 mg/mL（不溶） |
| 酸度系数(pKa) | 16.06±0.30(Predicted) |
| 形态 | 固体 |
| 颜色 | 浅黄至黄色 |
Macroautophagy/autophagy
CA-5f (0-40 μM, 6 hour) concentration- and time-dependently elevates the level of LC3B-II (a marker to monitor autophagy) and SQSTM1 protein both in A549 cells and HUVECs.
CA-5f (20 μM, 6 hours) inhibits the degradation of autophagosomes when treated alone or in combination Bafilomycin A1 (100 nM) or Chloroquine (30 μM) in A549 cells and HUVECs.
CA-5f (20 μM) neither impairs the hydrolytic function nor the quantity of lysosomes.
CA-5f (20 μM, 96 hours) inhibits the growth of A549 cells, and less cytotoxic to normal HUVECs.
Cell Viability Assay
| Cell Line: | A549, HUVECs |
| Concentration: | 20 μM |
| Incubation Time: | 96 hours |
| Result: | Exhibited more cytotoxicity against A549 cells compared with normal HUVECs. |
Western Blot Analysis
| Cell Line: | A549 cells and HUVECs |
| Concentration: | 0-40 μM |
| Incubation Time: | 6 hours |
| Result: | Elevated LC3B-II (a marker to monitor autophagy) and SQSTM1 protein levels in a concentration- and time-dependent manner. |
CA-5f (40 mg/kg, i.p., every 2 days for up to 30 days) is well tolerated, and potently inhibits the growth of tumor in nude mice bearing A549 lung cancer cells.
CA-5f (40 mg/kg, i.p.) suppresses autophagic flux and induces apoptosis in nude mice bearing A549 lung cancer cells.
| Animal Model: | Nude mice bearing A549 lung cancer cells |
| Dosage: | 40 mg/kg |
| Administration: | Injected via caudal vein, every 2 days for up to 30 days |
| Result: | Significantly suppressed tumor volume and weight in mice, increased the number of apoptotic cells in mice. |
安全信息
| 更新日期 | 产品编号 | 产品名称 | CAS号 | 包装 | 价格 |
|---|---|---|---|---|---|
| 2024/04/30 | HY-112698 | CA-5f | 1 mg | 419元 | |
| 2024/04/30 | HY-112698 | CA-5F CA-5f | 1370032-19-1 | 5mg | 1100元 |