2-硝基苯-beta-D-半乳糖苷
中文名称 | 2-硝基苯-beta-D-半乳糖苷 |
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中文同义词 | 2-硝基苯基-~-D-半乳糖苷, 98+%;2-硝基苯基-Β-D-吡喃半乳糖苷(植物源);2-硝基苯-Α-D-吡喃葡萄糖苷;邻硝基苯-Α-D-吡喃葡萄糖苷;邻硝基苯-Β-D-半乳糖苷(ONPG);邻硝基苯-Β-D-吡喃半乳糖苷(ONPG);邻硝基苯基半乳糖苷;2-硝基苯-Β-D-吡喃半乳糖苷(+4°C) |
英文名称 | 2-Nitrophenyl-beta-D-galactopyranoside |
英文同义词 | 2-Nitrophenyl hexopyranoside;2-Nitrophenyl-beta-delta-galactopyranoside;beta-D-Galactopyranoside, 2-nitrophenyl;beta-d-galactopyranoside,2-nitrophenyl;Galactopyranoside, o-nitrophenyl, beta-D-;o-Nitrophenyl beta-galactoside;2-Nitrophenyl-β-D-galactopyranoside;ONPG o-Nitrophenyl-?-D-galactoside |
CAS号 | 369-07-3 |
分子式 | C12H15NO8 |
分子量 | 301.25 |
EINECS号 | 206-716-1 |
相关类别 | 碳水化合物;专业试剂;酶类;中间体;氨基酸;核苷;糖类(碳水化合物);生化试剂;糖类;检测标志试剂;酶底物;糖类化合物;糖苷系列;多肽与蛋白;化学试剂;生化试剂-酶类;即用型溶液;分子生物试剂-多肽与蛋白;Substrates;Biochemistry;Galactose;Glycosides;Sugars;Enzyme substrates;Carbohydrates & Derivatives;substrate;酶活性检测;酶及活性检测;试剂;优势产品;其他原料;精细化工原料;化学原料;通用生化试剂-酶与辅酶;还原剂;化工中间体;其它;化学试剂 |
Mol文件 | 369-07-3.mol |
结构式 |
2-硝基苯-beta-D-半乳糖苷 性质
熔点 | 186-193 °C (dec.) |
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比旋光度 | -70 º (c=1, H2O) |
沸点 | 442.39°C (rough estimate) |
密度 | 1.3709 (rough estimate) |
折射率 | -68.8 ° (C=1, H2O) |
储存条件 | 2-8°C |
溶解度 | DMSO(微溶)、水(微溶、加热、超声处理) |
酸度系数(pKa) | 12.69±0.70(Predicted) |
形态 | 粉末 |
颜色 | 白色至微黄色 |
旋光性 (optical activity) | [α]20/D 70±1°, c = 1% in H2O |
水溶解性 | Soluble in water. |
BRN | 92207 |
InChIKey | KUWPCJHYPSUOFW-RMPHRYRLSA-N |
CAS 数据库 | 369-07-3(CAS DataBase Reference) |
NIST化学物质信息 | Galactopyranoside, o-nitrophenyl, beta-d-(369-07-3) |
The enzyme displays high hydrolysis ability for ONPG (100%) and moderate activity for its natural substrate lactose (25.7%). However, the hydrolysis ability of the enzyme towards all other chromogenic nitrophenyl analogues is very weak, indicating that Gal308 is a β-galactosidase with narrow substrate specificity. To investigate the kinetic parameters of recombinant enzyme, the Michaelis-Menten constants (K m ), turnover numbers (k cat ), and catalytic efficiencies (k cat /K m ) of Gal308 for ONPG and lactose are determined. The k cat and K m values are 464.7±7.8 s -1 and 2.7±0.3 mM for ONPG, and 264.2±2.1 s -1 and 7.1±0.8 mM for lactose, respectively. The k cat /K m value of the enzyme for ONPG (172.1 s -1 mM -1 ) is 4.6-fold higher than that for lactose (37.2 s -1 mM -1 ), which clearly demonstrated that the catalytic efficiency of Gal308 for ONPG is much higher than that for lactose.
安全信息
危险品标志 | Xi |
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危险类别码 | 36/37/38 |
安全说明 | 22-24/25-36-26 |
WGK Germany | 3 |
F | 3-10-21 |
海关编码 | 29400090 |
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