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| Phenosafranin Basic information |
Product Name: | Phenosafranin | Synonyms: | 3,7-DIAMINO-5-PHENYLPHENAZINIUM CHLORIDE;CI 50200;CI NO 50200;3,7-diamino-5-phenyl-phenaziniuchloride;nsc9855;Phenazinium,3,7-diamino-5-phenyl-,chloride;phenosafranine,chloride;2,8-DiaMino-10-phenyl-phenaziniuM Chloride | CAS: | 81-93-6 | MF: | C18H15ClN4 | MW: | 322.8 | EINECS: | 201-387-0 | Product Categories: | Amines;Aromatics;Mutagenesis Research Chemicals | Mol File: | 81-93-6.mol | ![Phenosafranin Structure](CAS/GIF/81-93-6.gif) |
| Phenosafranin Chemical Properties |
Melting point | 300 °C | Boiling point | 478.49°C (rough estimate) | density | 1.1738 (rough estimate) | refractive index | 1.6110 (estimate) | storage temp. | room temp | form | Solid | Colour Index | 50200 | color | Green to dark green | Merck | 13,7333 | BRN | 3642082 | CAS DataBase Reference | 81-93-6 |
Hazard Codes | Xi | Risk Statements | 36/37/38 | Safety Statements | 26-36 | WGK Germany | 3 | RTECS | SG1630000 |
| Phenosafranin Usage And Synthesis |
Chemical Properties | green crystalline powder | Uses | Phenosafranin is a bacterial stain used in microscopy, biological stain, and nuclear staining. | Definition | ChEBI: Phenosafranine is an organic chloride salt having 3,7-diamino-5-phenylphenazin-5-ium as the counterion. It is commonly used for staining Gram negative bacteria red in smears to contrast with the blue Gram positive organisms. It has a role as a fluorochrome, a histological dye and a photosensitizing agent. It contains a 3,7-diamino-5-phenylphenazin-5-ium. | Biochem/physiol Actions | Phenosafranin (PSF) is a red dye released from cells with intact plasma membrane. PSF at lower concentration is used to stain mitochondria supravitally. | Staining Procedures | Phenosafranin is used to distinguish living from dead cells. A 0.1 % aqueous solution, it is taken up by dead or abnormal cells immediately, while living cells remain unstained for at least 30 min. | Purification Methods | Crystallise the chloride from dilute HCl. It has UV with at 530nm in H2O. The picrate decomposes on heating and has a solubility of 0.0048% in H2O at 18o. [Beilstein 23 H 395, Beilstein 25 H 394, 25 I 654, 25 II 338, 25 III/IV 3050.] |
| Phenosafranin Preparation Products And Raw materials |
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