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| | 5-(Ethylthio)-1H-tetrazole Basic information |
| | 5-(Ethylthio)-1H-tetrazole Chemical Properties |
| Melting point | 72-80 °C(lit.) | | Boiling point | 81-82 °C | | density | 0.786 g/mL at 25 °C | | Fp | 42 °F | | storage temp. | 2-8°C | | solubility | soluble, clear, colorless | | form | buffered aqueous solution | | pka | 3.89±0.10(Predicted) | | color | solid white | | biological source | rabbit | | InChIKey | GONFBOIJNUKKST-UHFFFAOYSA-N | | CAS DataBase Reference | 89797-68-2(CAS DataBase Reference) |
| | 5-(Ethylthio)-1H-tetrazole Usage And Synthesis |
| Chemical Properties | White crystals | | Application | 5-(Ethylthio)-1H-tetrazole is used a powerful activator in DNA and RNA syntheses. | | Preparation | 5-(Ethylthio)-1H-Tetrazole is prepared from the corresponding alkyl thiocyanate and sodium azide through a [2?+?3] cycloaddition under phase transfer catalytic conditions (eq 1). The ratio of the solvent (water to toluene) as well as the reaction temperature are important factors in obtaining preparative yields of the alkylthiotetrazoles. 5-Ethylthio-1H-tetrazole has also been prepared using DMF as solvent albeit in much lower yield.
 | | General Description | 5-(Ethylthio)-1H-tetrazole (ETT) is an efficient activator, that activates nucleoside phosphoramidites towards condensation with a nucleoside to form dinucleoside phosphates during oligonucleotide synthesis. | | Purification Methods | recrystallized from toluene. |
| | 5-(Ethylthio)-1H-tetrazole Preparation Products And Raw materials |
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