FLAG肽
| 中文名称 | FLAG肽 |
|---|---|
| 中文同义词 | DDDDK肽;L-LYSINE, L-Α-ASPARTYL-L-TYROSYL-L-LYSYL-L-Α-ASPARTYL-L-Α-ASPARTYL-L-Α-ASPARTYL-L-Α-ASPARTYL-;FLAG® PEPTIDE;FLAG PEPTIDE 试剂;化合物FLAG PEPTIDE;FLAG TAG多肽;FLAG肽 |
| 英文名称 | FLAG peptide |
| 英文同义词 | FLAG tag Peptide;FLAG PEPTIDE|DYKDDDDK|ASP-TYR-LYS-ASP-ASP-ASP-ASP-LYS;FLAG Tag Peptide，FLAG epitope;FLAG®FLAG PEPTIDE, >98%;FLAG peptide,DYKDDDDK,Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, >98%;(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-6-amino-2-[[(2S)-2-[[(2S)-2-amino-3-carboxypropanoyl]amino]-3-(4-hydroxyphenyl)propanoyl]amino]hexanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-carboxypropanoyl]amino]hexanoic acid;Inhibitor,FLAG peptide,inhibit |
| CAS号 | 98849-88-8 |
| 分子式 | C41H60N10O20 |
| 分子量 | 1012.97 |
| EINECS号 | |
| 相关类别 | 植物生化提取物;多肽;生物功能肽;目录多肽;peptides;EpitopeTags |
| Mol文件 | 98849-88-8.mol |
| 结构式 |  |
FLAG肽 性质
| 密度 | 1.470±0.06 g/cm3 (20 ºC 760 Torr) |
|---|---|
| 储存条件 | Keep in dark place,Sealed in dry,Store in freezer, under -20°C |
| 溶解度 | DMSO 中≥50.65 mg/mL；水中≥210.6 mg/mL；乙醇中≥34.03 mg/mL |
| 形态 | 冻干粉 |
| 颜色 | 白色至米白色 |
| 序列 | H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH |
| InChIKey | XZWYTXMRWQJBGX-OVIGALBNNA-N |
Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca 2+ . Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca 2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions.
纯度(HPLC) ≥98.0%
醋酸根含量≤12.0%
水分含量≤8.0%
肽含量≥80.0%
内毒素≤50EU/mg
氨基酸组成分析≤±10%