JC-171 (JC171)
中文名称 | JC-171 (JC171) |
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中文同义词 | |
英文名称 | JC-171 (JC171) |
英文同义词 | JC-171 (JC171);Benzamide, 5-chloro-N-[2-[4-[(hydroxyamino)sulfonyl]phenyl]ethyl]-2-methoxy- |
CAS号 | 2112809-98-8 |
分子式 | C16H17ClN2O5S |
分子量 | 384.83 |
EINECS号 | |
相关类别 | |
Mol文件 | 2112809-98-8.mol |
结构式 | ![]() |
JC-171 (JC171) 性质
密度 | 1.401±0.06 g/cm3(Predicted) |
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储存条件 | Store at -20°C |
溶解度 | 二甲基亚砜:250 mg/ml(649.64 mM) |
形态 | 固体 |
酸度系数(pKa) | 6.43±0.69(Predicted) |
颜色 | 白色至米白色 |
JC-171 是选择性的 NLRP3 炎症小体抑制剂,抑制 LPS/ATP 诱导的巨噬细胞释放 IL-1β 的 IC50 值为 8.45 μM。
JC-171 (0-100 μM) blocks NLRP3 inflammasome activation and IL-1β production in primary macrophages dose dependently.
Cell Viability Assay
Cell Line: | J774A.1 murine macrophage cells |
Concentration: | 0-100 μM. |
Incubation Time: | 0.5 h (before LPS (1 μg/mL) treatment for 4.5 h). |
Result: | Inhibited the release of IL-1β in J774A.1 cells upon stimulation with LPS/ATP. |
JC-171 treatment delays the progression and reduces the severity of experimental autoimmune encephalomyelitis (EAE) in mouse.
Animal Model: | Mice immunized subcutaneously with 200 μg Myelin oligodendrocyte glycoprotein (MOG) 35–55 peptide emulsified in Complete Freund’s Adjuvant (CFA) on day 0 followed by injection of 200 ng of pertussis toxin. |
Dosage: | 100 mg/kg, 10 mg/kg. |
Administration: |
IP days 0, 1 and 2; and every other days thereafter (100 mg/kg).
Initiated when the clinical scores of individual mice have reached 1 (flaccid tail), and given every other day (10 mg/kg). |
Result: |
Efficiently suppressed EAE progression compared with vehicle treatment.
Resulted in a substantial decrease in the frequency of MOG 35–55 -specific Th17 cells in the spleens and spinal cords of EAE mice. |