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| | CREBtide Basic information |
| Product Name: | CREBtide | | Synonyms: | L-Arginine, L-lysyl-L-arginyl-L-arginyl-L-α-glutamyl-L-isoleucyl-L-leucyl-L-seryl-L-arginyl-L-arginyl-L-prolyl-L-seryl-L-tyrosyl-;CREBtide TFA | | CAS: | 149155-45-3 | | MF: | C73H129N29O19 | | MW: | 1716.99 | | EINECS: | | | Product Categories: | | | Mol File: | 149155-45-3.mol |  |
| | CREBtide Chemical Properties |
| storage temp. | Store at -20°C | | solubility | Soluble in DMSO | | form | Solid | | color | White to off-white |
| | CREBtide Usage And Synthesis |
| Biological Activity | CREBtide is a synthetic 13 amino acid peptide that acts as a PKA substrate. | | in vitro | delta-CREB is a spliced variant of cAMP response element binding protein (CREB). CREBtide (KRREILSRRPSYR), a synthetic peptide based on the phosphorylation sequence in delta-CREB. delta-CREB and CREBtide are tested as substrates of cAMP-dependent protein kinase (cAK). The apparent K m of CREBtide phosphorylation by cAK is 3.9 μM, which is 10-fold lower than that of Kemptide (K m =39 μM), the synthetic peptide substrate most often employed for cAK measurement. The V max values are 12.4 mumol/(min.mg) for CREBtide and 9.8 mumol/(min.mg) for Kemptide. The apparent K m of CREBtide phosphorylation by cGMP-dependent protein kinase (cGK) is 2.9 μM and the V max value is 3.2 mumol/(min.mg). Both delta -CREB and CREBtide are phosphorylated at a much slower rate by cGK as compared with cAK, implying that the high cAK/cGK specificity exhibits by delta-CREB is retained by the peptide. | | target | |
| | CREBtide Preparation Products And Raw materials |
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