多肽CREBtide
中文名称 | 多肽CREBtide |
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中文同义词 | PKA底物多肽CREBTIDE |
英文名称 | CREBtide |
英文同义词 | L-Arginine, L-lysyl-L-arginyl-L-arginyl-L-α-glutamyl-L-isoleucyl-L-leucyl-L-seryl-L-arginyl-L-arginyl-L-prolyl-L-seryl-L-tyrosyl-;CREBtide TFA |
CAS号 | 149155-45-3 |
分子式 | C73H129N29O19 |
分子量 | 1716.99 |
EINECS号 | |
相关类别 | |
Mol文件 | 149155-45-3.mol |
结构式 | ![]() |
多肽CREBtide 性质
储存条件 | Store at -20°C |
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溶解度 | 溶于二甲基亚砜 |
形态 | 固体 |
颜色 | 白色至米白色 |
PKA
delta-CREB is a spliced variant of cAMP response element binding protein (CREB). CREBtide (KRREILSRRPSYR), a synthetic peptide based on the phosphorylation sequence in delta-CREB. delta-CREB and CREBtide are tested as substrates of cAMP-dependent protein kinase (cAK). The apparent K m of CREBtide phosphorylation by cAK is 3.9 μM, which is 10-fold lower than that of Kemptide (K m =39 μM), the synthetic peptide substrate most often employed for cAK measurement. The V max values are 12.4 mumol/(min.mg) for CREBtide and 9.8 mumol/(min.mg) for Kemptide. The apparent K m of CREBtide phosphorylation by cGMP-dependent protein kinase (cGK) is 2.9 μM and the V max value is 3.2 mumol/(min.mg). Both delta-CREB and CREBtide are phosphorylated at a much slower rate by cGK as compared with cAK, implying that the high cAK/cGK specificity exhibits by delta-CREB is retained by the peptide.