H-GLY-ARG-GLY-ASP-SER-PRO-CYS-OH
| 中文名称 | H-GLY-ARG-GLY-ASP-SER-PRO-CYS-OH |
|---|---|
| 中文同义词 | L-CYSTEINE, GLYCYL-L-ARGINYLGLYCYL-L-Α-ASPARTYL-L-SERYL-L-PROLYL-;细胞粘附肽 |
| 英文名称 | H-Gly-Arg-Gly-Asp-Ser-Pro-Cys-OH |
| 英文同义词 | H-GLY-ARG-GLY-ASP-SER-PRO-CYS-OH;Glycyl-L-arginylglycyl-L-α-aspartyl-L-seryl-L-prolyl-L-cysteine;L-Cysteine, glycyl-L-arginylglycyl-L-α-aspartyl-L-seryl-L-prolyl-;L-Cysteine,glycyl-L-arginylglycyl-L-α-aspartyl-L-seryl-L-prolyl-;Cell adhesion peptide |
| CAS号 | 91575-26-7 |
| 分子式 | C25H42N10O11S |
| 分子量 | 690.73 |
| EINECS号 | |
| 相关类别 | 标准品 |
| Mol文件 | 91575-26-7.mol |
| 结构式 |  |
H-GLY-ARG-GLY-ASP-SER-PRO-CYS-OH 性质
| 密度 | 1.67±0.1 g/cm3(Predicted) |
|---|---|
| 酸度系数(pKa) | 3.24±0.10(Predicted) |
GRGDSPC is conjugated to acrylated dextran via thiol-acrylate reaction to regulate the interactions of human mesenchymal stem cells (hMSCs) with the photocrosslinkable hydrogels. To determine the conjugation kinetics and efficiency of GRGDSPC peptide to DEX-MAES16, various GRGDSPC concentrations (i.e., 5, 10 and 20 mg/1 g DEX-MAES16) are conjugated to the acrylated Dextran (DEX) macromer over time (0.25, 0.5, 1 and 3h) in PBS at pH 7.8 and the free thiol groups of unreacted peptides are quantified using Ellman’s assay. In addition, the reaction kinetics of the thiol-peptide to acrylated (DEX-MAES16) and methacrylated (DEX-HEMA16) macromers are compared. As early as 15 min conjugation, with 5, 10 and 20 mg of GRGDSPC peptide/1 g modified DEX, the peptide conjugation efficiencies with DEX-MAES are 105.40, 94.10 and 87.45%, respectively, while for the reaction with the DEX-HEMA they are 0.73, 15.78 and 18.42%, respectively. After 1h, the GRGDSPC conjugation with DEX-MAES is completed with the peptide concentration of 10 mg, but only 35.66% of the thiol groups of the peptide react with DEX-HEMA. The reaction kinetics are also monitored at 3 h of conjugation, and all of the 20 mg GRGDSPC peptide reacts with acrylated DEX compared to only 32.53% for the methacrylated DEX at this time point.