Company Name: |
Sigma-Aldrich
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Tel: |
021-61415566 800-8193336 |
Email: |
orderCN@merckgroup.com |
Products Intro: |
Product Name:Protamine sulfate salt from herring CAS:9007-31-2 Purity:Grade III, histone, free(Millon test) Package:1G Remarks:P4505-1G
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Company Name: |
Beijing OKA biological technology co., LTD
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Tel: |
010-010-010-62971590 18548936886 |
Email: |
jack@oka-vip.com |
Products Intro: |
Product Name:Protaminesulfatesalt,fromherring CAS:9007-31-2 Purity:BR Package:1g;5g
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PROTAMINE SULFATE GRADE III FROM HERRING manufacturers
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| PROTAMINE SULFATE GRADE III FROM HERRING Basic information |
Product Name: | PROTAMINE SULFATE GRADE III FROM HERRING | Synonyms: | PROTAMINE SULFATE GRADE III FROM HERRING;protamine sulfate from herring;Protamine sulfate salt herring;clupeine;protamine sulfate salt from herring;PROTAMINE SULFATE GRADE III FROM HERRING USP/EP/BP | CAS: | 9007-31-2 | MF: | | MW: | 0 | EINECS: | 232-726-0 | Product Categories: | | Mol File: | Mol File | |
| PROTAMINE SULFATE GRADE III FROM HERRING Chemical Properties |
pka | 7.4-8.0; pKb 2.9-3.3(at 25℃) | Merck | 13,2449 |
WGK Germany | 3 | RTECS | UK9450000 | F | 21-10 |
| PROTAMINE SULFATE GRADE III FROM HERRING Usage And Synthesis |
Uses | May be used for the removal of DNA from protein samples or for the purification of DNA binding proteins. It may be used as an alternative to polybrene in retroviral-mediated gene transfer. | Biochem/physiol Actions | Protamine in the form of solid lipid nanoparticles (SLN) promoted transfection with plasmid DNA more efficiently and with less cytotoxicity than comparable SLNs composed of Esterquat-1. | Purification Methods | Protamine sulfate is a strongly basic protein (white powder, see pK) used to precipitate nucleic acids from crude protein extracts. It dissolves to the extent of 1.25% in H2O. It is freely soluble in hot H2O but separates as an oil on cooling. It has been purified by chromatography on an IRA-400 ion-exchange resin in the SO 4 form and is washed with dilute H2SO4. Eluates are freeze-dried under high vacuum below 20o. This method is used to convert proteamine and protamine hydrochloride to the sulfate. [UV: Rasmussen Hoppe Seyler's Z Physiol Chem 224 97 1934, Ando & Sawada J Biochem (Tokyo) 49 252 1961, Felix & Hashimoto Hoppe Seyler's Z Physiol Chem 330 205 1963.] |
| PROTAMINE SULFATE GRADE III FROM HERRING Preparation Products And Raw materials |
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