硫酸耐尔蓝
中文名称 | 硫酸耐尔蓝 |
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中文同义词 | 5-氨基-9-(二乙基氨基)苯并[α]吩恶嗪-7-翁硫酸盐(2:1);硫酸耐尔蓝;硫酸尼罗蓝;耐尔蓝;耐尔兰A;尼罗蓝A;耐尔蓝A(>65%,BS);耐尔蓝A (硫酸耐尔蓝、耐尔兰、尼罗蓝A、5-氨基-9-(二乙基氨基)苯并[Α]吩恶嗪-7-翁硫酸盐(2:1)、硫酸尼罗蓝) |
英文名称 | CI 51180 |
英文同义词 | Nile Blue A, certified, pure 10GR;Resistance Ireland;Nile Blue A, Pure;Bis[5-amino-9-(diethylamino)benzo[a] phenazoxonium] Sulfate;5-amino-9-(diethylamino)benzo [a]phenoxazinium Sulfate;5-amino-9-(diethylamino)-benzo(a)phenoxazin-7-iusulfate(2:1);9-(diethylamino)-5-imino-5h-benzo(a)phenazoxazinsulfate(2:1);Benzo[.alpha.]phenoxazin-7-ium,5-amino-9-(diethylamino)-,sulfate(2:1) |
CAS号 | 3625-57-8 |
分子式 | C20H20N3O5S- |
分子量 | 414.46 |
EINECS号 | 222-832-5 |
相关类别 | 血液学和组织学;色素类;染色剂和染料;生化试剂;化学试剂;生化试剂-色素类;通用生化试剂-生物染色剂与化学指示剂;Phenoxazine;marker |
Mol文件 | 3625-57-8.mol |
结构式 |
硫酸耐尔蓝 性质
熔点 | >300 °C (dec.)(lit.) |
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储存条件 | room temp |
溶解度 | 易溶于水、乙醇 |
色指数 | 51180 |
形态 | 粉末 |
颜色 | 深绿色到黑色 |
酸碱指示剂变色ph值范围 | 10.1(BLUE)-11.1(RED) |
水溶解性 | Soluble in ethanol, and water (50 g/L) at 25°C. |
最大波长(λmax) | 633 nm |
生物领域应用 | Detecting microorganisms; treating virus infectious diseases; photodynamic therapy |
Nile blue A is a basic oxazine dye which is soluble in water and ethyl alcohol. Nile blue A is a satisfactory stain for PHB granules in bacteria and is in fact superior to Sudan black B for this purpose. Poly-p3-hydroxybutyrate granules exhibits a strong orange fluorescence when stained with Nile blue A. Nile blue A appears to stain many more PHB granules than Sudan black B does and is not as easily ished from the cell by decolorization procedures. Nile blue A is used as a stain for polyhydroxyalkanoic acid-accumulating microorganisms or to detect polyhydroxyalkanoic acids in microorganisms. Escherichia coli cells that do not accumulate detectable polyhydroxyalkanoic acids can be stained with Nile blue A and that this staining is sufficient for identifying these cells in fluorescence-activated cell sorting (FACS) experiments. Nile blue A staining does not affect either surface display of peptides or specific labeling of these peptides by a second fluorescence. Staining E. coli for flow cytometry using Nile blue A is an easy-to-handle and low-cost alternative to other fluorescent dyes or the intracellular expression of, for example, green fluorescent protein. Nile blue A is one of the most studied benzophenoxazine dyes, as a potent photosensitizer for photodynamic therapy. The dye when administered intravenously disperses throughout the body by circulating through blood and is taken up by most cells that emphasize its interaction with various biomolecule.
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