荧光素
中文名称 | 荧光素 |
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中文同义词 | 荧光橙红;黄 7 [CI 45350:1];荧光素,水溶;荧光酯;萤光橙黄;萤光红;萤光黄;萤光素 |
英文名称 | Fluorescein |
英文同义词 | Fluorescein, C.I. 45350, synthesis grade;Fluorescein, C.I. 45350, extra pure;FluoresceinC.I.45350;Fluorescein, laser grade, pure, 99%;Fluorescein, pure;Fluorescein,highpuritystandard;D&CYellow#7;Spiroisobenzofuran-1(3H),9-9Hxanthen-3-one, 3,6-dihydroxy- |
CAS号 | 2321-07-5 |
分子式 | C20H12O5 |
分子量 | 332.31 |
EINECS号 | 219-031-8 |
相关类别 | 滴定;分析试剂;标准品;小分子抑制剂;基因组学和分子诊断;色素类;生物染色剂;生化试剂;Aromatics;FUNDUSCEIN;Fluorescent;Xanthene;化合物;化学试剂;生化试剂-色素类;化工原料;大化工;化工材料;有机试剂;通用生化试剂-生物染色剂与化学指示剂;染料;试剂盒;日化品原料;有机化工原料;E-FFluorescent Probes, Labels, Particles and Stains;Fluorescein and Derivatives;Fluorescent Labels;Stains and Dyes;Stains&Dyes, A to;Fluorescent Labels & Indicators;Heterocycles;Intermediates & Fine Chemicals;Pharmaceuticals |
Mol文件 | 2321-07-5.mol |
结构式 |
荧光素 性质
熔点 | 320 °C(lit.) |
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沸点 | 429.44°C (rough estimate) |
密度 | 1.2739 (rough estimate) |
折射率 | 1.5000 (estimate) |
储存条件 | room temp |
溶解度 | 不溶于水、乙醚、苯、氯仿;溶于乙醇、甲醇、丙酮、乙酸乙酯、N,N-二甲基甲酰胺 |
色指数 | 45350 |
形态 | 粉末 |
酸度系数(pKa) | 2.2, 4.4, 6.7(at 25℃) |
颜色 | 红色到橙色 |
酸碱指示剂变色ph值范围 | Pink uorescence (4.0) to green uorescence (6.0) |
水溶解性 | insoluble |
最大波长(λmax) | 493.5nm, 496nm, 460nm, 515nm |
Merck | 14,4159 |
BRN | 94324 |
稳定性 | 稳定的。易燃。与强氧化剂不相容。 |
主要应用 | Organic light-emitting diode, nanoparticles, liquid crystal display, oil products, lip make-up, nucleic acid synthesis, amplification, sequencing and cloning, diagnosis of diabetic retinopathy, detecting yeast, multidrug resistance, proteins, antiHCV antibodies, target genes, enzymatic activity, nerve agent, nucleic acid sequences, imaging lung cancer, prostate cancer |
生物领域应用 | Detecting chromosomal aberration; diagnosing corneal diseases; marking/detecting insects; as antitumor agent; as antihistaminic agent; use in agriculture and plant cultivation; use in cosmetics; use in ophthalmology; as a substrate for measuring α-amylases activity, elastases activity, esterases activity, β-glucuronidases activity, horseradish peroxidases activity, kinases activity, monoamine oxidases (MAOs) activity, phosphatases activity, phospholipases activity, proteases/proteinases activity, telomerases activity activity |
LogP | 2.980 (est) |
CAS 数据库 | 2321-07-5(CAS DataBase Reference) |
EPA化学物质信息 | Fluorescein (2321-07-5) |
Fluorescein is nearly inactive as a photodynamic sensitizer. Fluorescein consequently is almost useless as a photodynamic sensitizer, but it is of great value in fluorescence immunoassays.
The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent.
荧光素外观呈橙黄或浅橙红色结晶粉末。熔点314-316℃(分解)。溶于热乙醇、冰醋酸、碱溶液,呈绿色萤光,不溶于水、苯、氯仿和乙醚。
荧光素是汞溴红的中间体,可用作萤光光度分析试剂,测定氯、溴、碘、硫离子;氧化-还原指示试剂;生物染色剂。
荧光素可用邻苯二甲酸酐与间苯二酚在硫酸参与下反应制得。
安全信息
危险品标志 | Xi |
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危险类别码 | 43-36-36/37/38 |
安全说明 | 22-24/25-37/39-26-36/37/39-27 |
WGK Germany | 3 |
RTECS号 | LM5075000 |
F | 10-21 |
TSCA | Yes |
海关编码 | 32042000 |
毒害物质数据 | 2321-07-5(Hazardous Substances Data) |
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