四甲基罗丹明-4-碘乙酰胺
| 中文名称 | 四甲基罗丹明-4-碘乙酰胺 |
|---|---|
| 中文同义词 | 四甲基罗丹明-4-碘乙酰胺 |
| 英文名称 | Tetramethylrhodamine-5-iodoacetamidedihydroiodide(5-TMRIA) |
| 英文同义词 | 9-[2-Carboxy-4-[(2-iodoacetyl)amino]phenyl]-3,6-bis(dimethylamino)xanthylium inner salt;Tetramethylrhodamine-4-iodoacetamide;Tetramethylrhodamine-5-iodoacetamide dihydroiodide;5-TMRIA [TetraMethylrhodaMine-5-IodoacetaMide Dihydroiodide];VINNHWDBTRUVKY-UHFFFAOYSA-N |
| CAS号 | 114458-99-0 |
| 分子式 | C26H24IN3O4 |
| 分子量 | 569.4 |
| EINECS号 | |
| 相关类别 | 荧光探针，标记，颗粒和染色 |
| Mol文件 | 114458-99-0.mol |
| 结构式 |  |
四甲基罗丹明-4-碘乙酰胺 性质
Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) 是一种选择性巯基反应染料，用于通过半胱氨酸残基对蛋白质进行非特异性标记。Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) 可用于共价标记 DNA 片段。
Tetramethylrhodamine-5-iodoacetamide (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 7.5 mM dye) shows different results for labeling carbonic anhydrase and myoglobin. At lower dye concentrations (up to 40:1 dye:thiol ratio) fluorescence labeling of only the carbonic anhydrase band is observed. However,as concentration increases, fluorescence labeling of both carbonic anhydrase and the lower myoglobin band is observed, indicating nonspecific labeling at these dye:thiol ratios.