Isopropyl Beta-D-1-Thiogalactopyranoside Iptg
Price | $2 |
Package | 20kg |
Min. Order: | 1kg |
Supply Ability: | 10000kg |
Update Time: | 2022-09-23 |
Product Details
Product Name: Isopropyl Beta-D-1-Thiogalactopyranoside Iptg | CAS No.: 367-93-1 |
Min. Order: 1kg | Purity: 98 |
Supply Ability: 10000kg | Release date: 2022/09/23 |
Product Name: IPTG
Synonyms: Isopropyl β-D-Thiogalactoside
CAS: 367-93-1
EINECS No.: 206-703-0
Purity: ≥ 99%
Properties: white or white powder, dissolved in water was clear and colorless liquid
Molecular formula: C9H18O5S
Molecular Weight: 238.30
Isopropyl β-D-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator.
IPTG, unlike allolactose, is not hydrolyzable by β-galactosidase, its concentration therefore remains constant in an experiment. For induction, a sterile, filtered 1 M solution of IPTG is typically added by 1:1000 dilution into a logarithmically growing bacterial culture, however, different final concentration of IPTG may also be used.
IPTG is an effective inducer of protein expression in the concentration range of 100 μM to 1.0 mM. Concentration used depends on the strength of induction required, as well as the genotype of cells or plasmid used. If lacIq, a mutant that over-produces the lac repressor, is present, then a higher concentration of IPTG may be necessary.
In blue-white screen, IPTG is used together with X-gal. Blue-white screen allows colonies that have been transformed with the recombinant plasmid rather than a non-recombinant one to be identified in cloning experiments.
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