荧光基因编码的Ca2+指示剂(GECO)是由阿尔伯塔大学的Robert Campbell实验室开发的。R-GECO可以与绿色荧光cADDis、cAMP传感器或我们的向上和向下DAG传感器配对,以同时检测活细胞中的Ca2+和环AMP或Ca2+和二酰基甘油。BacMam递送系统能够在包括iPSC衍生的心肌细胞和神经元在内的多种细胞类型中实现一致和可控的表达。
艾美捷 Montana Molecular-红色GECO Ca2+测定试剂盒#U0600R包含的材料:
Green Borealis 抗arrestin 生物传感器,BacMam 表达系统
未修饰的抗利尿激素2型受体(V2R),BacMam 表达系统
丁酸钠,500mM 水溶液
对照受体:血管紧张素II型1受体(AT1R),BacMam 表达系统
对照激动剂:血管紧张素II,5mM 水溶液
![61.png 61.png](https://www.labbase.net/upload/202406/27/202406271549585704.png)
HEK 293细胞中的多重DAG和Ca²+反应。痕量描述了对30μM Carbachol(n=16孔)R-GECO()的平均响应;绿色DAG(m)。
Montana Molecular-红色GECO Ca2+测定试剂盒相关产品:
#D2040G Borealis-B2AR
#D0231G 绿色膜靶向FMP cADDis cAMP
#D2070G Borealis-5-HT2AR
#U0200G 绿色上调cADDis cAMP检测试剂盒
文献参考:
X. Chen, et al. A PACAP-activated network for secretion requires coordination of Ca2+ influx and Ca2+ mobilization. bioRxiv. January 2024.
C. Amos, et al. Membrane lipids couple synaptotagmin to SNARE-mediated granule fusion in insulin-secreting cells. Molecular Biology of the Cell. December 2023.
J. Wu, et al. Interaction Between HCN and Slack Channels Regulates mPFC Pyramidal Cell Excitability and Working Memory. bioRxiv. March 2023.
M. Thomas, et al. Optically activated, customizable, excitable cells. PLOS One. December 2020.
L. Liu, et al. Diacylglycerol kinases regulate TRPV1 channel activity. Journal of Biological Chemistry. April 2020.
S. Hoare, et al. A kinetic method for measuring agonist efficacy and ligand bias using high resolution biosensors and a kinetic data analysis framework. Nature Scientific Reports Feb 2020.
Montana Molecular产品主要包括GPCR检测、细胞应激和毒性相关检测工具,为客户提供了长时间内观察候选药物对细胞过程影响的能力。艾美捷科技是Montana Molecular的中国代理商,为科研工作者提供优质的产品与服务。
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