QA-Bio-甲基化板（LC-PERMET-96）是可扩展的，可以用于1到96个样本的数量。将未用的试剂盒组分和未用的反应板孔存放在推荐的储存条件下，以便日后进一步的样本准备。
注意：由于对碘甲烷/甲基碘的运输限制，无法将这一组分随试剂盒提供。建议从您当地的化学品供应商处购买纯度≥99.0%（气相色谱法）的甲基碘。甲基碘的同义词：碘甲烷，CAS编号：74-88-4。
艾美捷QA-Bio-过甲基化试剂盒（LT-PERMET-VP96）组成：
96 well Permethylation plate 1 Plate
Dimethyl sulfoxide (DMSO) 40.0 ml
Dichloromethane (DCM) 60.0 ml
96 well Balance Plate 1 Plate
96 well Plate Lid 1 Lid / Mat
QA-Bio-过甲基化试剂盒用于糖苷的甲基化处理。甲基化可以稳定唾液酸，以便进行MALDI质谱分析，并有助于连接分析研究（MALDI-MS，ESI-MS）。
提高并增强质谱中糖苷的电离效率
增加糖苷的疏水性能，使其能够进行液相色谱-质谱（LC-MS）分析
可执行相对定量和绝对定量
稳定唾液酸基团
更容易确定分支和糖苷键的位置
能够在正离子模式下检测中性糖苷和酸性糖苷
时间表
步骤1：样品的过甲基化
a. Preparation of glycans As required
b. Addition of DMSO and sample to plate 15 minutes
c. Incubation 15 minutes
d. Addition of MeI to permethylation plate 15 minutes
e. Incubation 60 minutes
步骤2：全甲基化样品的提取和储存
a. Extraction of samples 3-4 hours
b. Drying permethylated samples 10-30 minutes
c. Addition of 70% MeOH for storage 10-20 minutes
QA-Bio-过甲基化试剂盒文献参考：
A. Dell; A. J. Reaseon; Kay-Hooi Khoo; M. Panico; A. McDowell and H. R. Morris Mass Spectrometry of Carbohydrate containing Biopolymer Methods in Enzymology 230: 108-132 (1994)
I.Ciucanu; C. E. Costello Elimination of Oxidative Degradation during the per-O-Methylation of Carbohydrates J. Am. Chem. Soc. 125: 16213-16219 (2003)
I.Ciucanu Per-O-Methylation reaction of structural analysis of carbohydrates by mass spectrometry Analytica Chimica Acta 576: 147-155 (2006)
I.Ciucanu; R. Caprita Per-O-Methylation of neutral carbohydrates directly from aqueous samples for gas chromatography and mass spectrometry analysis Analytica Chimica Acta 585: 81-85 (2007)
Shubhakar, A., Kozak, R.P., Reiding, K.R., Royle, L., Spencer, D.I.R., Fernandes, D.L., Wuhrer, M. Automated High-Throughput Permethylation for Glycosylation Analysis of Biologics using MALDI- TOF-MS Analytical Chemistry. acs.analchem. 6b01639 (2016).
艾美捷科技是QA-Bio的中国代理商，为科研工作者提供优质的产品与服务。
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