CD3是T细胞受体相关CD3复合物的成员。单克隆抗体CT-3识别鸡T细胞上至少三种分子量为20、19和17 kDa的多肽(其中两种是N-糖基化的)的复合物。该抗体还从溶解了洋地黄皂苷的T细胞裂解物中共沉淀出90 kDa的非J性多肽,该非J性肽可以还原为Mr 50和40 kDa的两种多肽。
SouthernBiotech-Pacific Blue™抗鸡CD3抗体用于流式细胞术检测。
艾美捷SouthernBiotech-小鼠抗鸡CD3-PACBLU(CT-3):
货号:8200-26
克隆:CT-3
同型:小鼠(BALB/c) IgG1κ
同型对照:小鼠IgG1-PACBLU (15H6)
特异性:鸡/鸽子 CD3
其他名称:T3/TCR复合物
免疫原:鸡胸腺细胞和Ig-血液单核细胞
结合物:PACBLU (太平洋蓝™)
缓冲液配方:含有< 0.1% 叠氮化钠的磷酸盐缓冲液
克隆性:单克隆
浓度:0.5 mg/mL
体积:0.2 mL
推荐储存:2-8°C;避免光照
应用:
流式细胞术 – 质量检测 1,5,10-18
免疫组织化学-冰冻切片 – 文献中有报道 2-8
免疫组织化学-石蜡切片 – 文献中有报道 9
免疫沉淀 – 文献中有报道 1
刺激 – 文献中有报道 1
![71.png 71.png](https://www.labbase.net/upload/202409/05/202409051553161587.png)
用小鼠抗鸡CD3-APC(SBCat.No.8200-11)和小鼠抗鸡CD8α-FITC(SB Cat.No.8220-02)对鸡外周血淋巴细胞进行染色。
SouthernBiotech小鼠抗鸡CD3-PACBLU(CT-3)文献参考:
1. Chen CH, Ager LL, Gartland GL, Cooper MD. Identification of a T3/T cell receptor complex in chickens. J Exp Med. 1986;164:375-80. (Immunogen, FC, IP, Stim)
2. Njenga MK, Dangler CA. Endothelial MHC class II antigen expression and endarteritis associated with Marek's disease virus infection in chickens. Vet Pathol. 1995;32:403-11. (IHC-FS)
3. Tanimura N, Sharma JM. Appearance of T cells in the bursa of Fabricius and cecal tonsils during the acute phase of infectious bursal disease virus infection in chickens. Avian Dis. 1997;41:638-45. (IHC-FS)
4. Zheng W, Yoshimura Y. Organ-specificity of estrogen effects on the induction of immunocompetent cells in the chicken. J Poult Sci. 2001;38:41-9. (IHC-FS)
5. Sheela RR, Babu U, Mu J, Elankumaran S, Bautista DA, Raybourne RB, et al. Immune responses against Salmonella enterica serovar enteritidis infection in virally immunosuppressed chickens. Clin Diagn Lab Immunol. 2003;10:670-9. (FC, IHC-FS)
6. Pantin-Jackwood MJ, Brown TP, Huff GR. Proventriculitis in broiler chickens: immunohistochemical characterization of the lymphocytes infiltrating the proventricular glands. Vet Pathol. 2004;41:641-8. (IHC-FS)
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