Human Integrin alpha 4 beta 7 (ITGA4&ITGB7) Heterodimer Protein, His Tag&Tag Free (MALS verified)
分子别名(Synonym)
Integrin alpha 4 beta 7,ITGA4&ITGB7
表达区间及表达系统(Source)
Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free (IT7-H52W4) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Glu 20 - His 723 (ITGB7) (Accession # P13612-1 (ITGA4) & P26010-1 (ITGB7)).
Predicted N-terminus: Tyr 34 (ITGA4) & Glu 20 (ITGB7)
蛋白结构(Molecular Characterization)
Human ITGA4&ITGB7 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB7, has a calculated MW of 111.4 kDa (ITGA4) & 83.7 kDa (ITGB7). The protein migrates as 55 kDa, 80 kDa, 100-120 kDa and 130-150 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
背景(Background)
整合素α4β7是一种整合素家族粘附受体,与α4β1(VLA4)和E-钙粘蛋白受体αEβ7共享亚基。整合素α4β7,对归巢肠粘膜至关重要,在Peyer斑块或肠系膜淋巴结的T细胞活化过程中被诱导。它的表达需要来自局部树突和基质细胞的信号,包括分泌的视黄酸。HIV-1包膜蛋白gp120与整合素α4β7的活性形式结合,这可以解释也可以不解释HIV-1病毒在肠道相关淋巴组织(GALT)中的浓度。整合素α4β7也可能参与急性肠移植物抗宿主病(GVHD)中的淋巴细胞运输。
关键字: 整合素a4b7;Integrin a4b7;a4b7重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group(股票代码:301080)是成立于2010年的跨国生物科技公司,是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球,横跨亚洲、北美洲、欧洲,在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家,与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。