Biotinylated Human PCSK9 Protein, Avitag™,His Tag (MALS verified)
分子别名(Synonym)
PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
表达区间及表达系统(Source)
Biotinylated Human PCSK9, Avitag,His Tag (PC9-H82E7) is expressed from human HEK293 cells. It contains AA Gln 31 - Gln 692 (Accession # Q8NBP7-1).
Predicted N-terminus: Gln 31
蛋白结构(Molecular Characterization)
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This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through noncovalent interactions and with a calculated MW of 13.8 kDa and 59.9 kDa respectively. The protein migrates as 17 kDa and 63-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
标记(Labeling)
Biotinylation of this product is performed using Avitag™ technology.
Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
蛋白标记度(Protein Ratio)
Passed as determined by the HABA assay / binding ELISA.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
![2.jpg 2.jpg](/SupplyImg1/2024-07-11/6385629367248194992006950.jpg)
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背景(Background)
前蛋白转化酶枯草杆菌蛋白酶/kexin 9型(PCSK9)也称为NARC1(神经细胞凋亡调节转化酶),是一种新发现的属于肽酶S8亚家族的枯草酶。小鼠PCSK9被合成为可溶性酶原,并在内质网中进行自催化分子内处理,导致其前肽的切割,该前肽与分泌的活性酶保持关联,具有宽的碱性最佳pH值。这种蛋白质在胆固醇稳态中起着重要的调节作用。PCSK9与低密度脂蛋白受体(LDLR)的表皮生长因子样重复序列A(EGF-A)结构域结合,诱导LDLR降解。PCSK9也可能在皮层神经元的分化中发挥作用。该基因的突变与一种罕见的常染色体显性家族性高胆固醇血症(HCHOLA3)有关。
关键字: PCSK9;PCSK9蛋白;PCSK9重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group(股票代码:301080)是成立于2010年的跨国生物科技公司,是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球,横跨亚洲、北美洲、欧洲,在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家,与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。