Human HMGB1 Protein, His Tag
分子别名(Synonym)
HMGB1,HMG1,HMG3,SBP-1
表达区间及表达系统(Source)
Human HMGB1, His Tag (HM1-H5220) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Glu 215 (Accession # P09429-1).
Predicted N-terminus: Met 1
蛋白结构(Molecular Characterization)
![1.jpg 1.jpg](/SupplyImg1/2024-08-06/6385853837505376501366756.jpg)
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.8 kDa. The protein migrates as 32 kDa and 35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>90% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
![2.jpg 2.jpg](/SupplyImg1/2024-08-06/6385853838201306519877470.jpg)
背景(Background)
高迁移率族蛋白B1(HMGB1)也称为高迁移率群蛋白1(HMG-1)和两性霉素,是由HMGB1、HMGB2和HMGB3三个成员组成的HMGB家族的成员。HMGB1是一种非组蛋白结构的染色体蛋白,普遍存在于所有脊椎动物的细胞核中,与双链DNA结合时没有序列特异性。炎症和损伤的机制与TLR4结合,TLR4介导HMGB1依赖性激活巨噬细胞细胞因子的释放。这使HMGB1处于无菌和感染性炎症反应的交叉点。HMGB1已被研究作为DNA疫苗佐剂和癌症治疗的靶点。
关键字: HMGB1;HMGB1蛋白;HMGB1重组蛋白;ACRO;百普赛斯;
百普赛斯集团ACROBiosystems Group(股票代码:301080)是成立于2010年的跨国生物科技公司,是为全球生物医药、健康产业领域提供关键生物试剂产品及解决方案的行业平台型基石企业。2021年在创业板上市。百普赛斯集团业务遍布全球,横跨亚洲、北美洲、欧洲,在中国、美国、瑞士等12个城市设有办公室、研发中心及生产基地。目前累计服务客户超6000家,与全球Top 20医药企业均建立了长期、稳定的合作伙伴关系。集团旗下拥有品牌ACROBiosystems百普赛斯、bioSeedin柏思荟、Condense Capital垦拓资本和ACRODiagnostics百斯医学等。