| 产品编号 |
产品名称 |
产品包装 |
产品价格 |
| P5493-1mg |
Recombinant Human FGF-21 |
1mg |
15444.00元 |
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
26291
|
Q9NSA1
|
E. coli
|
181aa
|
19.4kDa
|
－
|
|
About this Antibody
|
|
Name
|
Recombinant Human FGF-21 (Recombinant Human Fibroblast Growth Factor-21; rHuFGF-21)；重组人成纤维细胞生长因子21
|
|
Synonyms
|
FGF-21; fibroblast growth factor 21
|
|
Purity
|
>96% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5μg/ml, corresponding to a specific activity of >2.0×103IU/mg in the presence of 5μg/ml of rMuKlotho-β and 10μg/ml of heparin.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
|
|
Endotoxin
|
Less than 1EU/μg of rHuFGF-21 as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
Fibroblast growth factor-21 (FGF-21) belongs to the large FGF family which is encoded by the FGF-21 gene and it is specifically induced by HMGCS2 activity. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure and they are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-21 stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 expression (but not SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB. FGF-21 contains a 28a.a. signal sequence and a 181a.a. mature region but show limited binding to heparin. In addition, Mature human FGF-21 respectively shows 81%a.a. identity to murine and rat FGF-21, and is known to be active on murine cells.
|
|
Amino Acid Sequence
|
HPIPDSSPLL QFGGQVRQRY LYTDDAQQTE AHLEIREDGT VGGAADQSPE SLLQLKALKP GVIQILGVKT SRFLCQRPDG ALYGSLHFDP EACSFRELLL EDGYNVYQSE AHGLPLHLPG NKSPHRDPAP RGPARFLPLP GLPPALPEPP GILAPQPPDV GSSDPLSMVG PSQGRSPSYA S
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
P5493-5μg
|
Recombinant Human FGF-21
|
5μg
|
|
P5493-25μg
|
Recombinant Human FGF-21
|
25μg
|
|
P5493-100μg
|
Recombinant Human FGF-21
|
100μg
|
|
P5493-1mg
|
Recombinant Human FGF-21
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。