| 产品编号 |
产品名称 |
产品包装 |
产品价格 |
| P7239-25μg |
Recombinant Human DHH C23II |
25μg |
1152.00元 |
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
50846
|
O43323
|
E. coli
|
177aa
|
19.9kDa
|
－
|
|
About this Antibody
|
|
Name
|
Recombinant Human DHH C23II (Recombinant Human Desert Hedgehog Cys23IleIle; rHuDHH C23II)；重组人沙漠刺猬因子C23II
|
|
Synonyms
|
DHH; desert hedgehog; desert hedgehog (Drosophila) homolog; desert hedgehog homolog; desert hedgehog protein; HHG-3MGC35145
|
|
Purity
|
>96% by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 15-45μg/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2μm filtered concentrated solution in 10mM PB, pH6.0, 300mM NaCl.
|
|
Endotoxin
|
Less than 1EU/μg of rHuDHH C23II as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
|
|
Category
|
Others
|
|
Background
|
Desert hedgehog protein (DHH), also named HHG-3, is belonging to the hedgehog (Hh) family that are highly conserved proteins which are widely represented throughout the animal kingdom. This protein is predicted to be made as a precursor that is autocatalytically cleaved and the biologically active form of Hh molecules corresponds to approximately the N-terminal one half of the precursor molecule. The N-terminal portion is soluble and contains the signaling activity while the C-terminal portion is involved in processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. DHH is essential for a variety of patterning events during development as intercellular signal. It may function as a spermatocyte survival factor in the testes and play an important role in testes development. Mature human DHH shares 97% amino acid sequence identity with murine DHH.
|
|
Amino Acid Sequence
|
IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
P7239-5μg
|
Recombinant Human DHH C23II
|
5μg
|
|
P7239-25μg
|
Recombinant Human DHH C23II
|
25μg
|
|
P7239-100μg
|
Recombinant Human DHH C23II
|
100μg
|
|
P7239-1mg
|
Recombinant Human DHH C23II
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。