Йоднитротетразолий хлорид
Йоднитротетразолий хлорид атрибут
| Температура плавления: |
240 °C (dec.)(lit.) |
| температура хранения: |
2-8°C |
| растворимость: |
метанол: вода (1:1): 50 мг/мл горячий, очень слабо мутный, очень темно-желтый |
| форма: |
Порошок, кристаллы и/или куски |
| цвет: |
От белого до не совсем белого |
| Растворимость в воде: |
растворимый |
| Чувствительный: |
Light Sensitive |
| λмакс: |
248nm |
| БРН: |
4093224 |
| Стабильность:: |
Стабильный. Несовместим с сильными окислителями. |
| Биологические приложения: |
Bacterial vaginosis diagnosis assay; dehydrogenase enzyme assay; diagnostic assay; food and beverage analytes assays; microbial growth assay; detecting bacteria,yeast,fungi,gamma-hydroxybutyric acid (GHB),microbial growth; measuring niacin,bacterial respiratory activity,superoxide dismutase;treating cancer |
| InChI: |
InChI=1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1 |
| ИнЧИКей: |
JORABGDXCIBAFL-UHFFFAOYSA-M |
| SMILES: |
[N+]1(=NC(C2C=CC=CC=2)=NN1C1C=CC(I)=CC=1)C1=CC=C([N+]([O-])=O)C=C1.[Cl-] |
| Справочник по базе данных CAS: |
146-68-9(CAS DataBase Reference) |
| FDA UNII: |
XY3JA5594E |
| Система регистрации веществ EPA: |
2H-Tetrazolium, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-, chloride (146-68-9) |
Йоднитротетразолий хлорид химические свойства, назначение, производство
Описание
Iodonitrotetrazolium (INT) (chloride) is a monotetrazolium salt used as an indicator dye. It is reduced to an insoluble formazan that is used as a vital dye or indicator of cellular redox activity. Reduction commonly results from the activity of dehydrogenases, although non-enzymatic electron transfer reactions can occur in the presence of an intermediate electron acceptor. INT is commonly used to measure the respiratory activity of microorganisms in a variety of contexts.
Химические свойства
slightly yellow powder
Использование
Iodonitrotetrazolium chloride has been used for the staining of cells. It has been used as a coupling agent in the enzymatic assay of D-arabinitol.
Определение
ChEBI: An organic chloride salt having iodonitrotetrazolium as the counterion.
Безопасность
Iodonitrotetrazolium chloride could cause skin irritation, serious eye irritation, and may cause respiratory irritation.
Методы очистки
Recrystallise the chloride from H2O, aqueous EtOH or EtOH/Et2O. Alternatively, dissolve it in the minimum volume of EtOH and add Et2O; or dissolve it in hot H2O (charcoal), filter and precipitate it by adding conc HCl. Filter the solid off and dry it at 100o. Its solubility in H2O at 25o is 0.5%, and in hot MeOH/H2O (1:1) it is 5%. [Fox & Atkinson J Am Chem Soc 72 3629 1950, Beilstein 26 III/IV 1776.]
Йоднитротетразолий хлорид препаратная продукция и сырье
сырьё
препарат
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