ANTI-MYOSIN LIGHT CHAIN KINASE

Monoclonal anti-myosin light chain kinase antibody has been used in:

• immunohistochemistry
• immunoblot (diluted 1:10,000) analysis
• flow cytometry

Monoclonal Anti-Myosin Light Chain Kinase (mouse IgG2b isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Myosin light chain kinase (MLCK) is a calcium/calmodulin-dependent enzyme (approx.160 kD). It is expressed in skeletal, cardiac, smooth and mammalian non-muscle cells. MLCK is encoded by the MYLK1 gene. This gene encodes telokin, non-muscle and the smooth muscle isoforms of MLCK. MLCK gene is mapped to the human chromosome location 3q21.1.

Myosin Light Chain Kinase (MLCK) has a pivotal role in phosphorylating myosin regulatory light chains and hence facilitates its interaction with actin filaments for smooth muscle contraction. Monoclonal anti-myosin light chain kinase antibody can be used for studying the function of the enzyme and its interactions with other cell components. It can also be used in microarray and immunoprecipitation. Mouse anti-myosin light chain kinase antibody reacts specifically with myosin light chain kinase present in smooth muscle of chicken gizzard, turkey gizzard, and pig stomach. This product also shows reactivity for myosin light chain kinase of non-muscle cells like cultured fibroblasts.