ANTI-HUMAN IGA (ALPHA CHAIN), PEROXIDASE

Anti-Human IgA (α-chain specific) Peroxidase antibody produced in goat has been used in:

• Enzyme linked immunosorbent assays (ELISA) at dilution of 1:3,000 and 1: 1,600
• Tissue anti-transglutaminase antibody (tTG) assay at a dilution of 1:1,000
• Dot blot: a minimum working dilution of 1:80,000 was determined in a direct chemiluminescence assay using 10ng human IgA/dot. Luminol plus enhancer was used as substrate.
• Immunohistology using formalin-fixed, paraffin-embedded sections at a dilution of 1:100
• Transcytosis assay of dimeric IgA (D-IgA)
• Western Blotting

Anti-Human IgA (a-chain specific)-peroxidase antibody is isolated from goat anti-human IgA antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins, which do not specifically bind to the alpha chain of human IgA.Goat antihuman IgA is conjugated to peroxidase and purified to remove unconjugated material. Specificity of the peroxidase conjugated anti-human IgA is determined by immunoelectrophoresis (IEP) and cross-reactivity of the conjugate is determined by enzyme linked immunoassays (ELISA).

IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa and prevent the commensal microorganisms from invading the intestines.