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ACS Synthetic Biology

ACS Synthetic Biology

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Biosynthesis of 1,3-Propanediol via a New Pathway from Glucose in Escherichia coli

Published:14 June 2023 DOI: 10.1021/acssynbio.3c00122 PMID: 37316976
Mingda Li, Yang Zhang, Jingchuan Li and Tianwei Tan*, 

Abstract

1,3-Propanediol (1,3-PDO), an important dihydric alcohol, is widely used in textiles, resins, and pharmaceuticals. More importantly, it can be used as a monomer in the synthesis of polytrimethylene terephthalate (PTT). In this study, a new biosynthetic pathway is proposed to produce 1,3-PDO using glucose as a substrate and l-aspartate as a precursor without the addition of expensive vitamin B12. We introduced a 3-HP synthesis module derived from l-aspartate and a 1,3-PDO synthesis module to achieve the de novo biosynthesis. The following strategies were then pursued that included screening key enzymes, optimizing the transcription and translation levels, enhancing the precursor supply of l-aspartate and oxaloacetate, weakening the tricarboxylic acid (TCA) cycle, and blocking competitive pathways. We also used transcriptomic methods to analyze the different gene expression levels. Finally, an engineered Escherichia coli strain produced 6.41 g/L 1,3-PDO with a yield of 0.51 mol/mol glucose in a shake flask and 11.21 g/L in fed-batch fermentation. This study provides a new pathway for production of 1,3-PDO.

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Related products
Procduct Name CAS Molecular Formula Supplier Price
1,3-Propanediol 504-63-2 C3H8O2 634 suppliers $9.00-$1400.00
D-Glucose monohydrate 5996-10-1 C6H14O7 294 suppliers $47.30-$6717.78
VITAMIN B12 13408-78-1 C63H88CoN14O14P 119 suppliers Inquiry
ESCHERICHIA COLI 24 suppliers $257.00-$3911.12
L-aspartic acid, sodium salt 17090-93-6 C4H8NNaO4 9 suppliers Inquiry
Glutamate-Oxaloacetate Transaminase (GOT) 2 suppliers $192.00-$428.00

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