D-Methionine synthesis
- Product Name:D-Methionine
- CAS Number:348-67-4
- Molecular formula:C5H11NO2S
- Molecular Weight:149.21
1509-92-8
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348-67-4
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Yield:348-67-4 1 μmol
Reaction Conditions:
with 4-amino-2,3-dimethyl-1-phenylpyrazolin-5-one;5'-ATGTGACCCACCTGCGGCACGAGGACC-3';catalase;horseradish peroxidase;phenol at 4; for 0.0166667 h;Catalytic behavior;Kinetics;Enzymatic reaction;Reagent/catalyst;
Steps:
2.5. Enzyme activity assay and determination of kinetic parameters
N-d-AAase activity was measured using N-acetyl-d-methionine as a substrate. Concentration of reaction product was measured by applying a d-amino acid oxidase coupling with horse radish peroxidase. The detailed condition was as previously described [17]. Basically, N-d-AAase and N-acetyl-d-methionine were incubated at 4 ◦C. Then the mixture of d-amino acid oxidase and colorimetric solution containing peroxidase (10 U/ml), 4-aminoantipyrine (0.04 g/ml) and 0.8 l phenol was added. The OD at wavelength 520 nm was measured. One unit was defined as generation of 1 mol d-methionine in 1 min. Protein concentration was determined by the Bradford method and bovine serum albumin as the standard [32]. Kinetic parameters kcat and Km values were determined by fitting the initial rates as a function of substrate concentration to the Michaelis-Menten equation.
References:
Peng, I-Chen;Lo, Kai-Yin;Hsu, Chun-Hua;Lee, Chia-Yin [Process Biochemistry,2012,vol. 47,# 12,p. 1785 - 1790]
59-51-8
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348-67-4
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52811-68-4
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348-67-4
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44978-50-9
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348-67-4
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