D(+)-Xylose synthesis

Yield:-

Reaction Conditions:

with recombinant β-xylosidase/α-L-arabinofuranosidase/β-glucosidase from Cellulosimicrobium cellulans sp. 21 in aq. phosphate buffer at 30; pH=8; for 0.166667 h;Enzymatic reaction;

Steps:

2.6. Xylo-oligosaccharides degradation by CcXyl3A

General procedure: The hydrolytic activity of CcXyl3A on xylo-oligosaccharides was determined. The reaction mixture of 200 μl composed of 50 mM Na₂HPO₄-NaH₂PO₄ buffer (pH 8.0), 10 mg/ml xylobiose (X2), xylotriose (X3) or xylo-oligosaccharides mixture (XOS-70,Shandong Longli Co.) and 10 μg/ml purified CcXyl3A was incubated at 30 °C for 10 min. The hydrolytic products of xylobiose and xylotriose were determined by thin layer chromatography (TLC). The TLC plates were developed using ethyl acetate/methanol/water (16/6/1, v/v/v) as developing solvent. Products were visualized by spraying the plates with sulphuric acid/ethanol (5/95, v/v) followed by heating for 5 min at 105 °C in an oven. The hydrolytic products of xylo-oligosaccharides mixture were determined by Inertsil NH₂ column (DIKMA 4.6 × 250 mm, 5 μm) connected to an HPLC system (Shimadzu, Japan), eluted at the flow rate of 1.0 ml/min for 15 min with 65% acetonitrile (in distilled water, v/v), monitored by the refractive index detector.

References:

Yuan, Ye;Hu, Yanbo;Zhang, Han;Leng, Jiayi;Li, Fan;Zhao, Xuesong;Gao, Juan;Zhou, Yifa [Journal of Molecular Catalysis B: Enzymatic,2016,vol. 131,p. 65 - 72]