L-Glutathione synthesis
- Product Name:L-Glutathione
- CAS Number:27025-41-8
- Molecular formula:C20H32N6O12S2
- Molecular Weight:612.63
57564-91-7
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27025-41-8
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10102-43-9
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Yield:-
Reaction Conditions:
with GLUTATHIONE;8C12H6N9(3-)*12Cu(2+)*3H(1+)*3Cl(1-)*72H2O*12C3H7NO in water; for 16 h;Inert atmosphere;Reagent/catalyst;
Steps:
2.6. Reaction setup
All reactions described herein were carried out under inert, N2 gas, atmosphere, unless otherwise noted. GSNO and GSH solutions were prepared from Millipore H2O and solid GSNO or GSH powder under inert conditions (N2) in a 200mL round bottom flask. CuBTTri was weighed into a multi neck 100mL round-bottomed flask and oven dried overnight at 110°C. Following drying, the flask containing CuBTTri was placed under vacuum for 1h on a Schlenk line and backfilled with N2 (g) prior to reaction. GSNO and GSH solutions were then injected into the reaction flasks containing dry CuBTTri. Vigorous bubbling in the solution was established. Reaction flasks were wrapped in aluminum foil to prevent exposure to light and reactions proceeded for a predetermined time. To quench the reaction once the reaction time had been reached, the exit needle was removed to stop bubbling and the supernatant was carefully decanted via a syringe, leaving the MOF particles in the flask. The quenched reaction solution was then kept cool and dark in a Cu-free glass vial under inert conditions (N2) or added directly to an NMR tube. The 1H NMR sample was prepared in a septa capped sample tube under inert conditions (N2) by injecting 0.5mL of reaction supernatant into the NMR tube along with 0.1mL of 20mM NaH2PO4 buffered D2O. An identical procedure as described was carried out for the reaction between GSNO and CuCl2. No unanticipated safety hazards were encountered over the course of all experiments. All reactions reported in the Results and discussion section of this work were performed in triplicate to obtain an average and standard deviation
References:
Tuttle, Robert R.;Rubin, Heather N.;Rithner, Christopher D.;Finke, Richard G.;Reynolds, Melissa M. [Journal of Inorganic Biochemistry,2019,vol. 199,art. no. 110760]
70-18-8
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27025-41-8
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86810-11-9
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27025-41-8
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27025-41-8
435 suppliers
$5.00/25mg