429653-73-6
中文名称
429653-73-6
英文名称
Y16
CAS
429653-73-6
分子式
C24H20N2O3
分子量
384.43
MOL 文件
429653-73-6.mol
更新日期
2025/01/10 15:39:02
429653-73-6 结构式
基本信息
中文别名
化合物Y16G蛋白偶联RHO GEFS抑制剂(Y16)
英文别名
Y16Y16, >98%
Y16, 429653-73-6
4-[[3-[(3-Methylphenyl)methoxy]phenyl]methylene]-1-phenyl-3,5-pyrazolidinedione
3,5-Pyrazolidinedione, 4-[[3-[(3-methylphenyl)methoxy]phenyl]methylene]-1-phenyl-
所属类别
生物化工：激动剂抑制剂物理化学性质
密度1.273±0.06 g/cm3(Predicted)
储存条件2-8°C
溶解度DMSO：可溶5mg/mL，澄清（加热）
酸度系数(pKa)8.84±0.20(Predicted)
形态粉末
颜色淡黄色至深橙色
常见问题列表
生物活性
Y16是G蛋白偶联Rho GEFs抑制剂。它能阻止LARG和Rho的结合，Kd值为80 nM。靶点
| Target | Value |
|
RhoGEFs
() |
体外研究
Y16 (10-30 μΜ; 24 hours; NIH 3T3 cells) could inhibit RhoA-GTP formation induced by serum dose dependently and is specific for RhoA.
Y16 (10-30 μΜ; 24 hours; NIH 3T3 cells) efficiently inhibits serum or SDF-1α-induced phospho-MLC and phospho-FAK formation, which are downstream of RhoA.
Cell Viability Assay
| Cell Line: | NIH 3T3 cells |
| Concentration: | 10 μΜ, 30 μΜ |
| Incubation Time: | 24 hours |
| Result: | Inhibited RhoA-GTP formation induced by serum dose dependently and was specific for RhoA. |
Western Blot Analysis
| Cell Line: | NIH 3T3 cells |
| Concentration: | 10 μΜ, 30 μΜ |
| Incubation Time: | 24 hours |
| Result: | Inhibited serum or SDF-1α-induced phospho-MLC and phospho-FAK formation, which were downstream of RhoA. |