Pyridoxalhydrochlorid Chemische Eigenschaften,Einsatz,Produktion Methoden
R-Sätze Betriebsanweisung:
R20/21/22:Gesundheitsschädlich beim Einatmen,Verschlucken und Berührung mit der Haut.
R36/37/38:Reizt die Augen, die Atmungsorgane und die Haut.
S-Sätze Betriebsanweisung:
S24/25:Berührung mit den Augen und der Haut vermeiden.
S22:Staub nicht einatmen.
S36:DE: Bei der Arbeit geeignete Schutzkleidung tragen.
S26:Bei Berührung mit den Augen sofort gründlich mit Wasser abspülen und Arzt konsultieren.
Beschreibung
Pyridoxal hydrochloride is a hydrochloride salt formed by combining pyridoxal with hydrochloric acid. It is also a derivative of pyridinium salt and vitamin B6. It has important roles in E. coli metabolites, Saccharomyces cerevisiae metabolites, cofactors, human metabolites and mouse metabolites. It can be used as a calibrator for liquid chromatography of food samples, a component of culture media for lactic acid bacteria (LAB) and foodborne pathogens (FBP) (component of arginine decarboxylase and lysine decarboxylase).
Chemische Eigenschaften
White to off-white crystalline powder
Verwenden
Pyridoxal hydrochloride is used in labeling of amino acids for their detection. It finds application in neurotransmitters, sphingolipids and aminolevulinic acid. It is also used as a nutritional agent. It is also applied in research studies like biochemical, drugs and in media supplements.
Definition
ChEBI: Pyridoxal hydrochloride is a hydrochloride obtained by combining pyridoxal with one molar equivalent of hydrochloric acid. It has a role as an Escherichia coli metabolite, a Saccharomyces cerevisiae metabolite, a cofactor, a human metabolite and a mouse metabolite. It is a hydrochloride, a pyridinium salt and a vitamin B6. It contains a pyridoxal(1+).
Allgemeine Beschreibung
Pyridoxal is a heterocyclic compound, weighing 167.2 Da. It is one of the natural forms of vitamin B
6. Pyridoxal is found to be less stable than pyridoxine, hence heating might result in the loss of its action. Pyridoxal has a wide variety of sources and is present in both plants and animals. Pyridoxal serves as an efficient precursor for coenzymes : pyridoxal phosphate and pyridoxamine phosphate.
Sicherheitsprofil
Poison by
intramuscular, intravenous, and
intraperitoneal routes. Moderately toxic by
ingestion and subcutaneous routes. When
heated to decomposition it emits very toxic
fumes of NOx and HCl. See also
ALDEHYDES.
Synthese
In
the 25mL reaction flask with agitator and thermometer, add successively
0.085g (0.5mmol) 2,2,6,6-tetramethyl piperidine-nitrogen-oxyradical,
0.29g (2mmol) cupric bromide, 0.40g (5mmol) pyridine and 2.06g (10mmol)
pyridoxinehydrochloride. Add
water 5mL, temperature is controlled at 30 DEG C, opens and stirs.Then
drip 2.0g (18mmol) 30% hydrogen peroxide, control temperature of
reaction at 30～35 DEG C simultaneously.After dropwising, insulation
continues to stir 1～2 hour at 30～35 DEG C, and gained reaction solution
reaches 99% (liquid-phase condition: chromatographic column is XDB-G8
250 × 4.6 mm 5um through Liquid Detection pyridoxol transformation
efficiency.Moving phase is methyl alcohol: buffer=15: 85; Buffer:0.04%
sodium pentanesulfonate is adjusted PH to 3 with Glacial acetic acid;
Detection wavelength is 284nm), reaction preference is 98%.
In
addition, get about 5mL reaction solution and directly go up silicagel
column, eluent is methylene dichloride: methyl alcohol=20: 1, obtain
final elutriant through gradient elution, and be concentrated into
dryly, can obtain white solidpyridoxalhydrochloride, fusing point 164.1-164.8 DEG C, nuclear-magnetism characterizes as follows: 1hNMR (400MHz, DMSO), δ: 8.27 (s, 1H, CHO), 6.63 (d, 1H, CH), 5.00-5.16 (m, 2H, CH2), 2.62 (s, 3H, CH3).
Because pyridoxalhydrochloride is unstable, difficult separation, reaction solution
then adds p-ethoxyaniline 1.37g (10mmol), treated yellow Schiff alkali
(IX) 2.10g that obtains without separating.The total recovery of
two-step reaction is 88%.
läuterung methode
Dissolve it in water and adjust the pH to 6 with NaOH. Set aside overnight to crystallise. The crystals are washed with cold water, dried in a vacuum desiccator over P2O5 and stored in a brown bottle at room temperature. The free base is then converted to the hydrochloride with one equivalent of HCl. [Fleck & Alberty J Phys Chem 66 1678 1962, Beilstein 21/13 V 44.]
Pyridoxalhydrochlorid Upstream-Materialien And Downstream Produkte
Upstream-Materialien
Downstream Produkte
